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杨树叶中Na~+的积累与其耐盐能力的关系

Relationship between Accumulation of Na~+ in Leaves and Salt Tolerance of Different Populus Genotypes

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【作者】 杨迎霞; 郏艳红; 聂莉莉; 张越; 陈受宜; 王景安; 刘仲齐;

【Author】 YANG Ying-Xia1,JIA Yan-Hong2,NIE Li-Li2,ZHANG Yue2,CHEN Shou-Yi3,WANG Jing-An1,,LIU Zhong-Qi2,1College of Chemistry and Biology,Tianjin Normal University,Tianjin 300384,China;2Tianjin Research Center of Agricultural Biotechnology,Tianjin 300192,China;3Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China

【机构】 天津师范大学化学与生命科学学院; 天津市农业生物技术研究中心; 中国科学院遗传与发育生物学研究所;

【摘要】 以受体杨树品种‘107号’和转基因杨树‘18-1’的一年生枝条为材料,采用Hoagland营养液水培方法,检测二者植株生长以及根、茎、叶中Na+和K+含量变化的结果表明,在含100mmol·L-1NaCl的Hoagland营养液中,二者的生长速度都受到明显抑制,但后者的受抑程度较小;叶中Na+含量呈持续增加趋势,9d后其叶中的Na+含量显著高于前者。在二者叶片枯萎程度相同的情况下,‘18-1’叶中的Na+含量是‘107号’的1.6倍左右。在4mol·L-1NaCl溶液中,它们的表皮细胞死亡率分别为42%和97%。说明后者比前者有更高的耐盐能力。

【Abstract】 Plant growth and changes of Na+ and K+ concentrations in leaves,stems or roots were investigated in 1-year-old cuttings from receptor poplar ’107’(Populus×euramericana ’Neva’) and transgenic poplar ’18-1’,which were exposed to salt stress in Hoagland solution.It was shown that the growth of both poplars were markedly inhibited in Hoagland with 100 mmol·L-1 NaCl addition,while ’18-1’ was less inhibited than ’107’.The concentration of Na+ in leaves was keeping increasing,and after 9 day culture Na+ concentration in the leaves of ’18-1’ was greatly higher than that of ’107’.The accumulation amount of Na+ in ’18-1’ leaves was about 1.6 times higher than that in ’107’ when their leaves displayed same necrosis degree under NaCl stress.The death ratio of epidermal cells was about 42% and 97% in ’18-1’ and ’107’ in distilled water with 4 mol·L-1 NaCl addition,respectively.These results indicated that salt tolerance of the transgenic poplar ’18-1’ was much higher than that of receptor poplar ’107’.

【关键词】 杨树; 耐盐; NTHK1; Na+; K+;
【Key words】 poplar; salt tolerance; NTHK1; Na+; K+;
【基金】 国家重点基础研究发展计划(2006CB100102);“十一五”国家科技支撑计划(2007BAD67B02)
  • 【文献出处】 植物生理学通讯 ,Plant Physiology Communications , 编辑部邮箱 ,2009年11期
  • 【分类号】S792.11
  • 【被引频次】5
  • 【下载频次】125
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