【摘要】 将采自辽宁兴城地区在生长期间具典型卷叶病症状的金星无核(Venus Seedless)葡萄品种休眠枝条,用RT-PCR检测4种葡萄卷叶伴随病毒(Grapevine leafroll-associated viruses,GLRaVs),扩增得到了葡萄卷叶伴随病毒2号(GLRaV-2)和葡萄卷叶伴随病毒3号(GLRaV-3)两种病毒的主要外壳蛋白(major coat protein,CP)基因的完整序列(GenBank登录号分别为FJ786017和FJ786016)。这表明该葡萄植株受到了GLRaV-2和GLRaV-3辽宁分离物(GLRaV-2-LN和GLRaV-3-LN)的复合侵染。根据检测结果,克隆了GLRaV-2-LN基因组3′端CPm(minor capsid protein)、p19(19-kDa protein)和p24(24-kDa protein)基因(GenBank登录号分别为FJ786018、FJ786019和FJ786018)。序列分析表明,GLRaV-3-LN的CP基因全长942 nt,与已报道的国内外其它分离物CP基因全序列相比,核苷酸序列同源性为89.8%~91.8%,由此推导的氨基酸序列同源性为94.9%~97.4%。GLRaV-2-LN的CP、CPm、p19和p24基因全长分别为597 nt、672 nt、486 nt和618 nt。与国外报道的几个分离物的相应蛋白基因全序列相比,核苷酸序列同源性分别为88.3%~100.0%、78.7%~99.9%、75.1%~99.4%和87.5%~99.5%;由此推导的氨基酸序列同源性分别为92.9%~100.0%、89.2%~100.0%、73.9%~99.4%和89.3%~99.0%。更多还原

【Abstract】 The dormant cutting of grapevine(Vitis vinifera cv.Venus Seedless) with typical leafroll symptom originated from Xingcheng region in Liaoning Province was detected for Grapevine leafroll-associated viruses(GLRaVs) by RT-PCR.The results showed that two complete sequences of major coat protein(CP) gene of GLRaV-2(GenBank accession number FJ786017) and GLRaV-3(FJ786016) were obtained,which indicated that the grapevine plant detected was co-infected with GLRaV-2 and GLRaV-3.The genes encoding CPm,p19 and p24 located in 3′genomic RNA of GLRaV-2 Liaoning isolate(LN) were then cloned and sequenced.The nucleotide sequence of GLRaV-3 CP was 942 nt,and sequence analysis showed that the identities of nucleotide sequence and deduced amino acid sequence among GLRaV-3-LN and other previously reported isolates including ten overseas and two domestic isolates were ranged from 89.8% to 91.8% and 94.9% to 97.4%,respectively.The nucleotide sequences of CP,CPm(FJ786018),p19(FJ786019) and p24(FJ786018) of GLRaV-2-LN were 597 nt,672 nt,486 nt and 618 nt in length,respectively.The relative sequence analysis showed that the nucleotide identities of CP,CPm,p19 and p24 among GLRaV-2-LN and other isolates reported were ranged from 88.3% to 100.0%,78.9% to 100.0%,75.1% to 99.4% and 87.5% to 99.5%,and the identities of deduced amino acids were 92.9 to 100.0%,89.2% to 100.0%,73.9% to 100.0% and 89.3% to 99.0%,respectively.更多还原