【摘要】 为探讨钙信号与犬肾上皮细胞(MDCK)活力之间的关系,采用MTT比色法观察了特异性钙调素拮抗剂-三氟拉嗪,胞内Ca2+螯合剂-BAPTA/AM以及Ca2+载体-A23187对MDCK细胞活力的影响。结果表明,12.5,25μmol/L三氟拉嗪和15μmol/L的BAPTA/AM作用72 h后分别使MDCK细胞活力降至对照组的51.4%,25.1%和34%,三氟拉嗪与BAPTA/AM对MDCK细胞活力的抑制作用随药物浓度增加和作用时间延长而增强;5μmol/L的A23187对MDCK细胞具有一定程度的刺激效应,但这种刺激效应随时间的延长而减弱;15μmol/L的BAPTA/AM可增强三氟拉嗪对MDCK细胞活力的抑制作用。说明Ca2+-钙调素系统在MDCK细胞生长和增殖过程中发挥重要作用。更多还原

【Abstract】 The objective of this study was to explore the relationship between calcium signals and viability of Mardin-Darby canine kidney(MDCK) cells in vitro.MTT colorimetric assay were used to detect the effects of specific calmodulin antagonists,trifluoperazine(TFP),as well as Ca2+ chelators,BAPTA/AM and calcium ionophore A23187 on the viability of MDCK cells in vitro.The results showed that 12.5 μmol/L TFP,25 μmol/L TFP and 15 μmol/L BAPTA/AM obviously decreased the viability of MDCK cells to 51.4%,25.1% and 34% of the control after 72 hours,respectively.TFP and BAPTA/AM inhibited the viability of MDCK cells in a dose-and or time-dependent manner,the higher concentration and the longer time,the more significant the inhibition effects.A23187(5 μmol/L) stimulated the cell viability in a certain extent,but the stimulatory effect was decreased with prolonged time.The presence of 15 μmol/L BAPTA/AM obviously enhanced the inhibition of TFP on the viability of the MDCK cells.The experimental findings presented here suggest that Ca2+-CaM system may be engaged in in the development and proliferation of MDCK cells to modulate the cell viability and behavior.更多还原