【摘要】 目的:应用功能分类基因芯片分析凉血化瘀方对急性肝衰竭大鼠细胞凋亡相关基因表达的影响,探讨其抗肝衰作用的分子机制。方法:腹腔注射GalN(500 mg.kg-1)+LPS(4.8μg.kg-1),造成大鼠急性肝功能衰竭,应用美国Super Array公司大鼠细胞凋亡Oligo基因芯片检测模型组和治疗组基因的差异表达。结果:模型组与正常组比较表达下调的基因有37条,表达上调的基因有10条;治疗组与模型组比较表达下调的基因有16条,其中诱导细胞凋亡基因有13条,表达上调的基因有12条,其中抗细胞凋亡基因7条。结论:凉血化瘀方能够调节细胞凋亡相关基因从而抑制肝细胞凋亡,主要是通过下调凋亡诱导相关基因的表达,同时也少量上调凋亡抑制基因的表达。更多还原

【Abstract】 Objective:To study the effect of the cooling blood to remove blood stasis formula on gene expression profiles of cell apoptosis in FHF rats by gene chip technology,and discuss the molecular mechanism of the formula in counteracting FHF（fulminant hepatic failure）.Method:Reproduce FHF rats models by D-GalN 500 mL·kg^-1 and LPS 4.8 μg·kg^-1 through intraperitoneal injection.Detect the differential expression between model group and drug treatment group by apoptosis gene chip（Oligo Apoptosis Microarray,Super Array Company）.Result:There were 37 down-regulated genes and 10 up-regulated genes in the model group compared with the normal group;there were 16 down-regulated genes,among which there are 13 apoptosis-induced genes,and 12 up-regulated genes in the medicine group compared with the model group.Conclusion:The formula can inhibit hepatocyte apoptosis by regulating apoptosis-related genes through down-regulating apoptosis-induced gene and slightly up-regulating apoptosis-inhibited genes.更多还原