【摘要】 应用组织学和免疫组织化学的方法,对17β-雌二醇(E2)影响鲻(Mugil cephalus)早期精巢的精子发生及其作用机制进行了研究。饲喂含15mg/kg和50mg/kg17β-雌二醇的饲料60d,以不添加17β-雌二醇的饲喂组为对照。结果表明,幼鲻投喂含E2饲料前,对照组和实验组精巢均属早期精巢,胞囊中原始的精原细胞占优势,Sertoli细胞紧贴精原细胞。投喂含E2饲料后1个月,低剂量组(15mg/kg)的4尾幼鲻均出现精原细胞的有丝分裂,有丝分裂细胞的数量多于高剂量组(50mg/kg),对照组精巢未见精原细胞的有丝分裂,说明E2能够诱发精原细胞的分裂。实验结束后投喂E2组精巢发育处在小生长期,可见精巢中有初级精母细胞存在于精小叶中,而对照组刚刚出现精原细胞的发育分化。用雌激素受体α和β亚型抗体进行免疫组织化学染色发现,对照组和实验组早期精巢中的精原细胞和sertoli细胞均显示强的免疫阳性反应,这为E2诱发精原细胞有丝分裂的作用机制提供了新的形态学证据。更多还原

【Abstract】 Using histological and immunohistochemical methods,the effects and mechanism of estradiol-17β(E2)on early spermatogenesis of grey mullet,Mugil cephalus were investigated. Experimental groups were fed with commercial diet containing 15 mg/kg(low dosage)and 50 mg/kg(high dosage)estradiol-17β for 60 days. Control group was fed with basic feed without additive. Results indicated that testises of experimental and control groups were both in the same developmental level at the beginning of the experiment,and the primordial spermatogonia absolutely predominated in cyst with sertoli cells clinging to spermatogonia. After 1 month feeding,mitosis in spermatogonia could be observed in experimental groups with mitosis cell number in low-dose group significantly more than that in high-dose group,and no mitosis was observed in control group. Above results showed E2 could hasten cell’ s division. At the end of the experiment, primary spermatocytes cound be found in seminiferous lobules of testis in experimental group. However,at the same time spermatogonia just began to differentiate in control group. Estrogen receptor α and β subtypes in the early testis of grey mullet were localized in spermatogonia and sertoli cells by using immunohistochemical method,which provided morphological evidence for deeply studying the mechanism of E2 in inducing spermotogonia’s mitosis.更多还原