【摘要】 目的:研究铜绿假单胞菌对亚胺培南的耐药机制。方法:取69株临床分离的耐亚胺培南铜绿假单胞菌(Imipenem-re-sistant pseudomon asaeruginosa,IRPA),经K-B法检测耐药性;用PCR法对铜绿假单胞菌亚胺培南耐药相关的金属酶IMP、VIM基因和外膜蛋白OprD2基因等3种主要耐药基因进行了检测与分析;结果:69株IRPA均为多重耐药菌,对阿米卡星、头孢哌酮/舒巴坦、头孢吡肟敏感性较高;检出金属酶IMP和VIM型金属酶基因阳性的分别有2株(2/69,2.9%)和4株(4/69,5.8%),外膜蛋白通道OprD2基因检测为缺失的有46株(46/69,66.7%)。结论:IRPA耐药情况严重。外膜蛋白通道OprD2基因缺失突变是本组铜绿假单胞菌对亚胺培南耐药的重要机制之一。更多还原

【Abstract】 Objective:To study the mechanisms of imipenem resistance in Pseudomonas aeruginosa.Methods:Drug resistance of 69 isolates of imipenem-resistant Pseudomonas aeruginosa(IRPA)were tested by Kirby-Bauer disk diffusion method;IMP and VIM genes of metallo-β-lactamases,and outer membrane protein D2(OprD2)gene of IRPA were detected and analyzed by PCR method.Results: All 69 isolates were multidrug resistant,and the sensitivity rates of AMK,CFS and FEP were highe.The positive rates for IMP gene and VIM gene were 2.9%(2/69)and 5.8%(4/69),respectively.46 of 69(66.7%)isolates presented with deletion for OprD2 gene. Conclusion:The drug resistance of IRPA is a serious issue.The deletion mutation of OprD2 gene is one of important mechanisms of imipenem resistance in these tested P.aeruginosa isolates.更多还原