【摘要】 【目的】研究采食水平对初产母猪妊娠早期孕酮分泌、胚胎存活和胚胎成纤维细胞生长因子受体-2(FGFR2)、孕酮受体(PGR)及胚胎和子宫内膜视黄醇结合蛋白4(RBP4)、叶酸结合蛋白(FBP)、子宫转铁蛋白(UF)基因表达的影响。【方法】母猪配种后随机分到3个组,按2.0倍、1.2倍和0.6倍维持需要供给饲粮(分别为采食高、中、低水平),妊娠12、25和35d屠宰母猪,收集血清、子宫液和胚胎、子宫组织,运用ELISA测定血清和子宫液中孕酮浓度,用RT-PCR测定目的基因在胚胎和子宫内膜中的表达。【结果】妊娠12、25和35d,血清孕酮浓度在采食高水平组极显著低于采食中、低水平组(P<0.01)。子宫分泌液孕酮浓度在妊娠12d,采食低水平组极显著高于采食高水平组(P<0.01),采食中水平组显著高于采食高水平组(P<0.05),妊娠25d各组差异不显著(P>0.05)。妊娠12d采食中水平组的活胚数显著高于采食高水平组(P<0.05),采食高、低水平间差异不显著(P>0.05)。妊娠各阶段,采食中水平组的胚胎FGFR2、RBP4以及子宫内膜RBP4、FBP、UFmRNA的相对表达量显著升高(P<0.05)。【结论】妊娠35d,以中水平采食量饲喂初产母猪,具有较高的活胚数,采食高水平或者严重限饲,引起孕酮分泌过高或过低,降低胚胎FGFR2、RBP4及子宫内膜RBP4、FBP、UFmRNA的表达,降低活胚数。更多还原

【Abstract】 【Objective】 Sixty-three Landrace×Yorkshire crossbred gilts were allotted to three treatment groups of high(H,2×maintenace),medium(M,1.2×maintenace),and low(L,0.6×maintenace) after mating to study the effects of feed intake level on progesterone secretion,embryonic survival and mRNA expression of fibroblast growth factor receptor 2(FGFR2),progesterone receptor(PGR) gene in embryo and retinol binding protein 4(RBP4),folate-binding protein(FBP),uteroferrin(UF) gene in embryo and endometria.【Method】 Gilts were slaughtered on day 12,25 and 35 of pregnancy,and serum,uterine flushings,embryos and endometria were collected.ELISA was adopted to determine the progesterone concentration in serum and uterine flushings,and RT-PCR was adopted to determine the genes expression in embryos and endometria.【Result】 Progesterone concentrations in serum were lower in H than M and L(P<0.01) on day 12,25,35 of pregnancy.In uterine flushings progesterone concentrations was lower in H than M and L(P<0.05,P<0.01) on day 12 of pregnancy,and no difference was observed on day 25 of pregnancy.Total viable embryos were greater in M than H group(P<0.05) on day 12 of pregnancy.No difference was observed between H and L group(P>0.05).Medium feed intake level significantly increased the relative mRNA expression of FGFR2 and RBP4 in embryo and RBP4,FBP,UF in endometria(P<0.05) on day 12,25 and 35 of pregnancy.【Conclusion】 Medium feed intake level had greater total viable embryos during day 1-35 of pregnancy in gilts.High feed intake level and restricted feeding induced higher or lower secretion of progesterone and decreased expression of FGFR2,RBP4 in embryo and RBP4,FBP,UF in endometrial,resulting in detrimental effects on the development of early embryo.更多还原