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细叶桉遗传多样性的ISSR分析

Genetic Diversity of Eucalyptus tereticornis by ISSR

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【作者】 张党权田华谢耀坚谭晓风黄青云谷振军曹家光曾艳玲

【Author】 ZHANG Dang-quan1,TIAN Hua1,XIE Yao-jian2,TAN Xiao-feng1, HUANG Qing-yun1,GU Zhen-jun1,CAO Jia-guang2,ZENG Yan-ling1(1.The Key Laboratory of Non-wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology,Changsha 410004; 2.China Eucalyptus Research Centre,SFA,Zhanjiang 524022)

【机构】 中南林业科技大学经济林育种与栽培国家林业局重点实验室国家林业局桉树研究开发中心

【摘要】 利用ISSR分子标记技术对细叶桉的遗传多样进行了分析.从100条随机引物中筛选出10条特异的用于细叶桉ISSR-PCR扩增引物,扩增出101条清晰可重复的条带,其中89条是多态条带,多态带百分率为86.1%.采用POPGENE软件,计算了种间的Nei氏遗传距离,并用UPGMA法构建了聚类分析树形图,分析了它们的遗传多样性和亲缘关系.

【Abstract】 The genetic diversity of Eucalyptus tereticornis was analyzed by ISSR technology.According to the improved ISSR-PCR system,10 primers were selected out of 100 random ones to yield specific bands with moderate band numbers,clear background and good reproducibility.Totally,101 specific and reproducible DNA bands were obtained,of which 89 are polymorphic bands(86.1% of polymorphism).Bio-software POPGENE 1.31 and NTSYS-pc were used to calculate the Nei genetic distance and produce the UPGMA dendrogram of genetic diversity and similarity.

【基金】 长沙市科技计划项目重点课题(K069083-22);湖南省教育厅科学研究项目(08C931)
  • 【文献出处】 中南林业科技大学学报 ,Journal of Central South University of Forestry & Technology , 编辑部邮箱 ,2009年05期
  • 【分类号】S792.39
  • 【被引频次】16
  • 【下载频次】138
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