【摘要】 以马尾松优良种子萌发后的幼苗的茎段为实验材料,研究了不同培养基类型、激素种类和激素质量浓度对愈伤组织诱导的影响.结果表明:改良GD培养基为诱导愈伤组织的最佳诱导培养基;改良GD+4 m g.L-12,4-D+2 m g.L-16-BA+500 m g.L-1CH+30 g.L-1蔗糖+8 g.L-1琼脂的组合为愈伤组织最佳增殖配方;暗培养20 d后平均直径能达到8.5 mm.这为马尾松幼苗茎段愈伤组织分化或进行遗传转化奠定了研究基础.更多还原

【Abstract】 Stems from Pinus massoniana seedlings were cultured on different kinds of media and hormones.Results show that the best basal medium for inducting callus is improved GD,namely,improved GD+4 mg·L-1 2,4-D + 2 mg·L-1 6-BA+ 500 mg·L-1CH+30 mg·L-1 sucrose+8 mg·L-1 agar;and that the average diameter of inducted callus can grow to 5.8 mm after 20 d dark culture.This study lays some scientific foundation for the differentiation of callus and genetic transformation.更多还原