【摘要】 目的观察家蝇3龄幼虫滞育前后血淋巴中蛋白质及组织匀浆脱氧核糖核酸(DNA)、核糖核酸(RNA)的变化,探讨家蝇幼虫滞育发生的生化机制。方法低温诱导家蝇3龄幼虫滞育,经SDS?聚丙烯酰胺凝胶电泳观察非滞育组及各滞育组血淋巴蛋白组分变化;紫外分光光度计测定非滞育组及各滞育组RNA和DNA含量。结果(1)滞育组和非滞育组家蝇幼虫蛋白条带数目无变化,只是滞育20d组的蛋白含量明显高于非滞育组,其中以85kd处最明显,光密度值为203475.69。(2)各滞育组DNA均比非滞育组明显降低;滞育10d组、15d组RNA明显降低,滞育20d组则明显升高;RNA/DNA在滞育10d组、15d组明显降低分别为0.598、0.546μg/mg,滞育20d组则明显升高为1.337μg/mg。结论滞育家蝇幼虫体内可能无滞育关联蛋白(DAP)的存在,RNA/DNA比值的降低和升高可作为家蝇幼虫滞育开始及滞育结束的信号。更多还原

【Abstract】 Objective To observe the change of protein in the hemlymph and DNA and RNA in the tissue from the 3rd stage larva of Musca domestica before and after diapause. To explore the biochemistry mechanism of M.domestica larvae diapause. Methods The diapause of the 3rd stage larva of M.domestica was inducted by low temperature.(1)The change of hemlymph protein in the diapause and the nondiapause groups were observed by polyacrylamide gel electrophoresis-SDS. (2) The RNA and DNA in the tissue of the diapause and the nondiapause groups were extracted and the contents were determined by ultraviolet spectrophotometer. Results (1) The numbers of protein straps in the diapause groups were similar to that in nondiapause group.But the content of the protein in the groups after diapause for 20 d was obviously higher than that in the nondiapause group. The optical density value was 203 475.69 at 85 kd.(2)The DNA content in all diapause groups was much lower than that of nondiapause group. The RNA content obviously decreased after diapause for 10 d and 15 d, but it obviously increased after diapause for 20 d. The contents of RNA and DNA after diapause for 10 d and 15 d decreased to 0.598, 0.546 μg/mg, but they increased to 1.337 μg/mg after diapause for 20 d. Conclusion Perhaps there was no diapause associated protein in diapause M.domestica larva. The decrease and increase of the proportion of RNA to DNA could be as the signal of beginning and ending of M.domestica larva diapause.更多还原