【摘要】 目的通过观察结缔组织生长因子(CTGF)在糖尿病大鼠肾脏中表达的动态改变,探讨CTGF在糖尿病肾病发生发展中的作用。方法尾静脉内注射链脲佐菌素(STZ)诱导糖尿病大鼠模型。采用RT-PCR和Western blot检测1个月、3个月和6个月时糖尿病大鼠肾脏组织中CTGF的mRNA和蛋白的表达。光镜下观察糖尿病大鼠不同时期肾脏组织形态学的改变。结果糖尿病大鼠1个月、3个月和6个月与同期对照大鼠相比,肾脏CTGF的mRNA表达上调,分别为对照组的1.56倍、2.05倍和3.74倍(P<0.01);同时蛋白表达亦上调,分别为对照组的2.48倍、2.93倍和5.82倍(P<0.01);此外,糖尿病6个月大鼠的CTGF mRNA和蛋白的表达明显高于糖尿病1个月和3个月大鼠的表达水平(P<0.01)。组织形态学观察表明,糖尿病大鼠3个月时肾脏出现基膜增厚、肾小球扩张等糖尿病肾病早期的病理改变,随着病程的延长,病理改变越严重;6个月时出现肾小球硬化、肾间质纤维化等晚期的改变。结论糖尿病大鼠早期肾脏已存在CTGF基因表达上调,在糖尿病肾病的发展过程中,CTGF基因表达上调仍持续存在。因此,CTGF基因表达上调在糖尿病肾病的发生发展中起着重要作用。更多还原

【Abstract】 Objective In order to explore the role of CTGF in the initiation and development of experimental diabetic nephropathy,the time course of CTGF gene expression was investigated in diabetic rat kidney.Methods Diabetic rats were induced by injection of streptozotocin (STZ) to the tail vein.CTGF mRNA and protein expression were determined by RT-PCR and western blot in diabetic rat kidney at 1 month,3 months and 6 months of experimental diabetes.The pathological changes of diabetic rats were examined by light microscopy.Results CTGF mRNA and protein expression levels were coordinately upregulated as early as 1 month and persisted over 6 months.Inspection of the renal tissue by light microscopy demonstrated glomerular hypertrophy and basement membrane thickening at 3 months and global glomerular sclerosis,interstitial fibrosis at 6 months.Conclusion Upregulated CTGF gene expression was found to occur before the initiation of diabetic nephropathy and last in the progress of diabetic nephropathy.This implies that the upregulated CTGF gene expression play an important role in the initiation and development of diabetic nephropathy.更多还原