【摘要】 目的研究"泛耐"铜绿假单胞菌中多种耐药基因的存在情况及耐药机制。方法采用聚合酶链反应(PCR)法检测22株"泛耐"铜绿假单胞菌的β-内酰胺酶基因、氨基糖苷类修饰酶(AMEs)基因、喹诺酮类耐药基因、耐消毒剂基因(qacE△1-sul1)和整合酶基因。结果PCR扩增结果显示22株菌中oprD2、blaCARB、gyrA、aac(6’)-Ⅰb、ant(2″)-Ⅰ和qacE△1-sul1基因均阳性;blaSHV、blaSPM、blaGIM、blaDHA、blaOXA-10、blaGES、blaPER、aac(3’)-Ⅰ和aac(6’)-Ⅱ基因均阴性;blaIMP、blaTEM、blaVEB、aac(3’)-Ⅱ、ant(3″)-Ⅰ、intⅠ1和blaVIM阳性率分别为95.5%、77.3%、13.6%、4.5%、4.5%、4.5%和4.5%。结论oprD2基因阳性说明这些泛耐菌株中外膜蛋白并未缺失,多种耐药基因同时存在是本研究中铜绿假单胞菌"泛耐"的根本原因。更多还原

【Abstract】 Objective To investigate resistance-associated genes of pan resistant Pseudomonas aeruginosa.Methods We extracted DNA of pan resistant Pseudomonas aeruginosa isolated from clinical patients and detected β-lactamase genes,aminoglycoside modification enzymes(AMEs) genes and resistant antiseptic(qacE△ 1-sul1)gene with polymerase chain reaction(PCR).Results PCR results showed that the positive ratios of aac(6′)-Ⅰ b,blaCARB,gyrA,oprD2,ant(2″)-Ⅰ and qacE△ 1-sul1 genes were all 100%;blaIMP,blaTEM,blaVEB,aac(3′)-Ⅱ,ant(3″)-Ⅰ,intⅠ 1 and blaVIM genes were 95.5%,77.3%,13.6%,4.5%,4.5%,4.5% and 4.5%,respectively.While aac(3′)-Ⅰ、 aac(6′)-Ⅱ 、 blaGES、 blaGIM、 blaOXA-10、 blaPER、 blaSPM、 blaSHV and blaDHA genes were all negative.Conclusions The positive oprD2 genes indicated that outer-membrane proteins still existed in these Pseudomonas aeruginosa.The pan resistance of these Pseudomonas aeruginosa accounted for available multi-kinds of resistance associated genes.更多还原