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吴茱萸提取物清除亚硝酸盐的作用

Removing Effect of Extracts on Nitrite from Evodia rutaecarpa(Juss.) Benth

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【作者】 甄攀史国茹周杏灿张利民

【Author】 ZHEN Pan,SHI Guo-ru,ZHOU Xing-can,ZHANG Li-min(College of Medicine,Hebei North University,Zhangjiakou 075000,Hebei,China)

【机构】 河北北方学院基础医学院

【摘要】 研究吴茱萸提取物清除亚硝酸盐(NO2-)的作用.通过NO2-的重氮化偶联反应产生偶氮染料,用分光光度法测定吴茱萸正丁醇提取物1、正丁醇提取物2、乙醚提取物对NO2-的清除作用.三种提取物及Vc清除亚硝酸根的IC50分别为405.0μg.mL-1、237.0μg.mL-1、9.8μg.mL-1、8.6μg.mL-1.正丁醇提取物1、2中总皂苷和总黄酮的含量分别为28.71%、31.27%和43.25%、44.31%.三种吴茱萸提取物均有一定的清除亚硝酸根的作用并呈量效关系;乙醚提取物清除NO2-的作用非常显著,与Vc作用相当,具有潜在的开发价值.

【Abstract】 The extract from Evodia rutaecarpa(Juss.) Benth was used for removing nitrite.NO2-was to be azo dyes through diazotization-coupling reaction,spectrophotometry was used to determine the removing effect on nitrites of the first and second extract with n-butanol and the extract with ether from Evodia rutaecarpa(Juss.) Benth.The IC50 of removing nitrites of these three extracts and Vc were 405.0,237.0,9.8,8.6μg·ml-1,respectively.The content of the saponin and Flavone in the first and second extract with n-butanol were 28.71%,31.27% and 43.25%,44.31%.These three extracts from Evodia rutaecarpa(Juss.) Benth can be used to remove nitrites.Especially the extract with ether,its effect on nitrites is same with Vc,and has potential development value.

【关键词】 吴茱萸提取物清除亚硝酸盐
【Key words】 Evodia rutaecarpa(Juss.) Benthextractremovenitrite
【基金】 河北省卫生厅医学科学研究重点课题(08385)
  • 【文献出处】 河北北方学院学报(自然科学版) ,Journal of Hebei North University(Natural Science Edition) , 编辑部邮箱 ,2009年06期
  • 【分类号】X703
  • 【被引频次】1
  • 【下载频次】175
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