【摘要】 目的研究慢性乙型肝炎患者人类白细胞抗原-DR基因(HLA-DRB1*03)与白细胞介素-18(IL-18)表达水平的相关性。方法采用PCR-SSP方法对153例慢性乙型肝炎患者与正常对照138例进行HLA-DRB1*03检测,并同时用双抗体夹心法检测IL-18表达水平。结果153例慢性乙型肝炎患者中HLA-DRB1*03携带者34例,携带率为22.22%,明显高于正常对照人群的携带率5.79%;HLA-DRB1*03阴性乙型肝炎患者IL-18平均表达水平明显高于阳性患者(P<0.05)。结论HLA-DRB1*03等位基因携带者对HBV感染具有易感性,HLA-DRB1*03(-)慢性乙型肝炎患者Th1因子表达水平高于HLA-DRB1*03(+)慢性乙型肝炎患者。更多还原

【Abstract】 OBJECTIVE To study the relationship between HLA-DRB1*03 genes and expression of cytokine IL-18 in chronic hepatitis B patients.METHODS A total of 153 chronic hepatitis B patients and 138 normal healthy people were detected.HLA-DRB1*03 alleles were conducted in all the patients with polymerase chain reaction-sequence specific primer(PCR-SSP),and IL-18 was detected by ELISA.RESULTS Thirty-four individuals with chronic hepatitis B patients were HLA-DRB1*03 genes positive,the frequency was 22.22%,which was higher than the frequency of HLA-DRB1*03 in heathy individuals.The average level of IL-18 in chronic hepatitis B patients with HLA-DRB1*03 negatives was significantly higher than in HLA-DRB1*03 positives.CONCLUSIONS HLA-DRB1*03 closely relates with susceptibility to chronic hepatitis B.In chronic hepatitis B patients,the average level of Th1 cytokines in HLA-DRB1*03 negatives is higher than that in HLA-DRB1*03 positives.更多还原