【摘要】 目的了解江西省中医院产超广谱β-内酰胺酶(ESBLs)的肺炎克雷伯菌的流行、耐药特点和基因型分布。方法用VITEK-32细菌鉴定仪/K-B法/双纸片法进行细菌鉴定/药敏试验/ESBLs初筛,用NCCLS1999年推荐方法确证ESBLs,并采用PCR方法对产ESBLs菌株进行基因分型。结果分离产ESBLs细菌42株,阳性率为35.0%;产酶株对各种抗菌药物的耐药率明显高于非产酶株,PCR分型:TEM型33株(78.6%),SHV型8株(19.0%),CTXM型29株(69.0%)。结论产ESBLs细菌具有多药耐药的特点;TEM型和CTXM型是医院产ESBLs肺炎克雷伯菌中流行的基因型。更多还原

【Abstract】 OBJECTIVE To investigate the epidemic,drug resistance and gene distribution of ESBLs-producing Klebsiella pneumoniae (KPN) from Jiangxi TCM Hospital. METHODS The susceptibility of KPN was detected by MIC PCR was used to detect ESBLs gene. RESULTS There were 42 strains with ESBLs isolated,the positive rate was 35.0%. The drug resistance rate of KPN with ESBLs was higher than that without ESBLs,PCR typing result:TEM 33 (78.6%),SHV 8 (19.0%) and CTXM 29 (69.0%). CONCLUSIONS The ESBLs-producing bacteria have multiple drug resistant genes;TEM and CTXM are the main drug resistant genes in our hospital.更多还原