【摘要】 目的通过两种采样方法以及不同培养基对呼吸机管路经机械物理消毒前后采样细菌培养,探讨呼吸机管路消毒效果监测最佳采样方法。方法运用稀释法和直接涂抹法分别对消毒前后呼吸机管路采样培养,同时采用普通营养琼脂和血培皿对照研究。结果消毒前后呼吸机管路用普通营养琼脂培养直接涂抹法细菌培养阳性率分别为100.0%、12.5%,采用稀释法阳性率分别为16.07%、0;用含血培养基培养消毒前后直接涂抹法细菌培养阳性率分别为100.00%、14.28%,采用稀释法阳性率分别为17.85%、0,差异有统计学意义(P<0.05),不同培养基对培养结果影响不大(P>0.05)。结论对呼吸机管路消毒效果监测运用直接涂抹采样法优于稀释法,能有效降低假阴性发生率,使消毒效果检测更准确。更多还原

【Abstract】 OBJECTIVE To explore disinfection effect of ventilator circuits and monitor the best one from two methods for sampling bacterial culture of pre-and post-mechanical physical disinfection ventilator circuit as well as different culture media.METHODS Pre-and post-disinfection ventilator circuits were sampled and cultured by the direct smudge method and the dilution method respectively which simultaneously the ordinary nutrient agar and the blood cultures used.RESULTS Bacterical positive rate of the direct smudge method was significantly higher than that of the dilution method in pre-and post-disinfection ventilator circuits(P<0.05),there was without difference in the different culture media(P>0.05).CONCLUSIONS The direct smudge method is superior to the dilution method in monitoring the disinfection effect of ventilator circuit,it can effectively reduce the false negative rate and make the disinfection effect examination to be more accurate.更多还原