【摘要】 目的了解分离于ICU的鲍氏不动杆菌(ABA)氨基糖苷类药物各种耐药相关基因存在状况,探讨ABA多药耐药机制。方法收集20株分离自2007年10月-2008年7月ICU患者临床标本的ABA,采用纸片扩散法测定32种抗菌药物的敏感性;采用PCR法检测armA、rmtA、rmtB、rmtC、rmtD、npmA、aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6′)-Iad、aac(6′)-Ⅰb、aac(6′)-Ⅱ、ant(3″)-Ⅰ、ant(2″)-Ⅰ16S rRNA甲基化酶与氨基糖苷类修饰酶基因。结果20株ABA菌株中检出aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6′)-Ⅰb、ant(3″)-Ⅰ4种氨基糖苷类修饰酶基因,阳性率分别为aac(3)-Ⅰ10%、aac(3)-Ⅱ15%、aac(6′)-Ⅰb 30%、ant(3″)-Ⅰ25%;未检出氨基糖苷类修饰酶新亚型-aac(6′)-Iad基因和16S rRNA甲基化酶基因。结论ABA对氨基糖苷类药物耐药情况严重、机制复杂,产氨基糖苷类修饰酶为主要原因之一。更多还原

【Abstract】 OBJECTIVE To investigate the antibiotic resistance of multi-resistant Acinetobacter baumannii(ABA) and distribution of aminoglycoside-modifying enzymes and 16S rRNA methylase genes in ICU in Yinzhou People′s Hospital in Ningbo. METHODS The samples of 20 ABA isolates were collected from Oct 2007 to Jul 2008 in ICU.K-B method was used to determine the sensitivity to 32 antibacterials and the aminoglycoside-modifying enzymes and 16S rRNA methylase genes were analyzed by polymerase chain reaction(PCR). RESULTS From 20 ABA isolates,8 strains carried aminoglycoside-modifying enzymes genes aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰb,and ant(3″)-Ⅰ,their positive rate was 10%,15%,30% and 25%,respectively;no strain carried 16S rRNA methylase genes. CONCLUSIONS The antibiotics resistance of A.baumannii is very serious in Yinzhou People′s Hospital in Ningbo.Aminoglycoside-modifying enzymes genes aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰb and ant(3″)-Ⅰ exist in multi-resistant A.baumannii widely.They would be the main causes of high drug-resistantce to aminoglycosides.更多还原