【摘要】 目的分析金属酶基因与整合子在嗜麦芽寡养单胞菌中存在情况。方法以细菌耐药鉴定系统及改良K-B法进行药敏试验,提取不同标本的DNA与质粒,以PCR反应扩增金属酶基因与整合子。结果L1、L2金属酶均存在于嗜麦芽寡养单胞菌染色体DNA及质粒DNA中,检测出少数菌株携带整合子Ⅰ、Ⅱ。结论L1、L2型金属酶基因为嗜麦芽寡养单胞菌多药耐药的重要原因之一,嗜麦芽寡养单胞菌能接受整合子,其耐药特性具有可获得性的。更多还原

【Abstract】 OBJECTIVE To study the existence of integron and metallo-β-lactamase in Stenotrophomonas maltophilia in our hospital.METHODS The antibiotic susceptibility was tested by K-B method.The genomic DNA and their plasmids were extracted.The integron and metallo-β-lactamase gene were amplified by polymerase chain reaction(PCR).RESULTS L1 and L2 genes were amplified in chromosomes and plasmids.Integrons Ⅰ and Ⅱ were detected in genomic DNA.CONCLUSIONS Existence of metallo-β-lactamase is one of reasons of multi-drug resistance in S.maltophilia.S.maltophilia can receive integrons and gain its multi-drug resistance from other strains.更多还原