【摘要】 目的:建立一种简便、实用、准确的鸡内金药材DNA分子标记鉴定方法,从而为建立鸡内金真伪鉴别方法奠定基础。方法:从G enbank数据库下载家鸡及3种常见伪内金源动物的m tDNA细胞色素b基因序列,经同位置序列比较,遵循引物设计原则选择一个差异点较多的片段,设计PCR引物(引物1,2),并使用该引物分别扩增家鸡、3种伪内金源动物,4种非伪内金源动物的DNA。结果:所设计的引物只扩增家鸡DNA,而不扩增其他动物DNA。结论:该引物具有特异性,可用于鸡内金药材的鉴定。更多还原

【Abstract】 Objective:To establish a convenient and accurate method of DNA molecular marker for the identification of corium stomachium galli.Method: Cytb mtDNA sequences of Gallus gallus domestica and three other species of poultry were downloaded from Genbank.Species-specific PCR primers were designed according to the differential DNA fragments of the cytb genes.PCR tests were performed with the DNAs extracted from G.gallus domestica,three other poultry species and domestic mammal animals.Result: The specific primers of G.gallus domestica could only amplify the cytb mtDNA of G.gallus domestica.Conclusion: The primers are specific to G.gallus domestica mtDNA and can used to discern Corium stomachium from the false medicine.更多还原