【摘要】 目的建立反相高效液相色谱法测定抗癌光敏剂舒他兰锌(Suftalan zinc)含量及有关物质。方法采用SHIMADZU SHIM-PACK VP-ODS色谱柱(4.6 mm×150 mm,5μm),以四氢呋喃-甲醇-N,N二甲基甲酰胺-水-三乙胺(150∶105∶755∶1 000∶1.4,磷酸调pH值6.5~7.0)为流动相,流速1.0 mL.min-1,紫外可见检测器于677 nm测定。结果RP-HPLC测定的线性范围为20.0~120.1 mg.L-1,相关系数r=0.999 9;最低检测限为0.048μg;样品溶液至少在3 d内稳定;日内精密度(RSD=0.70%)良好。结论采用反相高效液相色谱法测定抗癌光敏剂(舒他兰锌)含量及有关物质方法简便,结果准确。更多还原

【Abstract】 OBJECTIVE To establish a RP-HPLC method for the determination Suftalan zinc and its related substances.METHODS A separation was performed on a SHIM-PACK VP-ODS column(4.6 mm×150 mm,5 μm) and mobile phase was THF-CH3OH-DMF-H2O-TEA(150∶105∶755∶1 000:1.4,adjust pH to 6.5-7.0 using H3PO4) with a flow rate of 1 mL.min-1.The detective wavelength was 677 nm.RESULTS The calibration curve was linear in the concentration range of 20.0~120.1 mg.L-1(r=0.999 9).The LOD was 0.048 μg.The sample solution was stable within 3 d,intra-day validation(RSD=0.70%) was good.CONCLUSION The method to determine the content and its related substances of Suftalan zinc by RP-HPLC is easy to operate and the results of the determination is accurate.更多还原