【摘要】 目的:研究D101型大孔吸附树脂纯化油茶粕中油茶皂苷的工艺。方法:以油茶皂苷的含量为指标,考察D101型大孔吸附树脂的上样量、吸附容量和水溶性杂质、油茶皂苷洗脱的乙醇浓度及体积。结果:纯化工艺:D101型大孔吸附树脂的上样量为3 BV,吸附容量为566 mg·g^-1生药量,使用3BV蒸馏水和1 BV 20%乙醇洗脱水溶性杂质,3 BV85%乙醇洗脱油茶皂苷。结论: D101型大孔吸附树脂可较好地纯化油茶皂苷,该工艺简单,成本低,适用于工业化大生产。更多还原

【Abstract】 OBJECTIVE:To study thc purification technology of camellia saponin using D101 macroporous adsorption resin.METHODS:The loading volume of sample and the adsorption quantity of D101 macroporous adsorption resin and the concentration and volume of alcohol in eluting water -soluble foreign substances and total cemallia saponins were investigated taking the content of total cemallia saponins as index.RESULTS:The loading volume of sample of D101 macroporous adsorption resin was 3 fold that of resin D101（or 3 BV,resin bed volume） and its adsorption quantity was 566 mg·g^-1（crude drug）;3 BV distilled water and 1 BV 20%ethanol were used for the elution of the water-soluble foreign substances,and 3BV of 85%ethanol was used for the elution of total cemallia saponins.CONCLUSION:D101 macroporous resin has good purification efficacy for camellia saponins.The purification process is simple with low cost,and it is applicable for large - scale industrialized production.更多还原