【摘要】 以粳粳交高产106/长白9号的200个F2:3株系为作图群体,在0.15%Na2CO3溶液碱胁迫下,进行了水稻发芽率及其相对碱害率的鉴定评价,并以SSR标记构建的分子连锁图谱为基础,对水稻发芽率及其相对碱害率进行了数量性状基因座(QTL)检测。结果表明,在F3株系群中水稻发芽率及其相对碱害率均呈单峰接近正态的连续分布。共检测到碱胁迫下与水稻发芽率相关的QTL 7个,对表型变异的贡献率范围为4.05%～12.61%,其中位于第6染色体RM225-RM204区间的qGC-6和位于第9染色体RM219-RM3700区间的qGC-9对表型变异的贡献率分别为12.61%和10.85%。共检测到与水稻发芽率相对碱害率相关的QTL 6个,对表型变异的贡献率为4.82%～28.07%,其中位于第2染色体RM29-RM221区间的qRGC-2、位于第6染色体RM225-RM204区间的qRGC-6-1、位于第9染色体RM219-RM3700区间的qRGC-9和位于第12染色体RM260-RM3226区间的qRGC-12对表型变异的贡献率较大,分别为28.07%、15.35%、15.61%和18.91%,为主效QTL,但其相应的区间距离均较远,需要进一步深入研究。所检测的QTL增效等位基因主要表现为部分显性和超显性。更多还原

【Abstract】 The quantitative trait loci(QTLs) for germination capacity and relative alkali damage rate for germination capacity in rice under alkali stress(0.15% Na2CO3 solution) were identified by using an F2:3 population,which included 200 individuals and lines derived from a cross between two japonica cultivars Gaochan 106 and Changbai 9 with microsatellite markers.The germination capacity and its relative alkali damage rate showed a continuous and near normal distribution in F3 lines.Seven QTLs associated with the germination capacity under alkali stress were detected,which explained 4.05% to 12.61% of the observed phenotypic variances.qGC-6 and qGC-9 were located in RM225-RM204 and RM219-RM3700,respectively,which accounted for 12.61% and 10.85% of the observed phenotypic variation,respectively.Six QTLs correlated with relative alkali damage rate for germination capacity under alkali stress were detected.qRGC-2,qRGC-6-1,qRGC-9,and qRGC-12 were major QTLs located in RM29-RM221,RM225-RM204,RM219-RM3700,and RM260-RM3226,respectively,which explained 28.07%,15.35%,15.61% and 18.91% of the observed phenotypic variation,respectively.Given the long distances between the markers further research should be conducted.Most of the alleles were partially dominant or overdominant.更多还原