【摘要】 目的:观察探索学习对局灶性脑梗死大鼠神经颗粒素(Ng)及碱性成纤维细胞生长因子(bFGF)表达的影响。方法:健康雄性SD大鼠70只,其中60只电凝法造成右侧大脑中动脉阻断(MCAO)模型后,随机分为探索学习组(n=30)和对照组(n=30),假手术组(n=10)仅开颅不电凝大脑中动脉,居于标准笼。探索学习组和对照组分别于术后第1天、7天、14天、28天各组随机选取5只大鼠处死,假手术组分别于7天、28天时随机选取5只大鼠处死,即进行Ng及bFGF免疫组化染色,测定其在脑梗死灶周围皮质的表达情况。结果:探索学习组Ng及bFGF阳性神经元数在第7天、14天、28天明显多于对照组(P<0.01)。结论:探索学习能促进梗死灶周围皮质Ng及bFGF表达。更多还原

【Abstract】 Objective: To study the effects of learning on neurogranin (Ng) and basic fibroblast growth factor(bFGF) expressions in peri-infarction cortex of) rats after unilateral local cerebral infarction. Method:Seventy male Sprague- Dawley rats were adopted. After the models of MCAO were established by electric coagulation successfully, rats were randomly divided into learning group (n=30, living in maze cages), control group (n=30, every 5 rats as a subgroup living in large standard cages) and Other 10 rats didn′t receive electric coagulation at middle cerebral artery, as sham group living in standard cage. At the 1st, 7th, 14th, 28th d after MCAO, in learning group and control group 5 rats were randomly sacrificed separately. In sham group 5 rats were randomly sacrificed separately at the 7th, 28th d after operation. The expressions of bFGF and Ng in peri -infarction cortex were examined by immunohistochemistry staining. Result: The number of Ng and bFGF labeled cells in learning group was significomtly more than those in control group at 7th 14th and 28th day(P<0.01). Conclusion: Learning could promote expressions of Ng and bFGF in peri-infarction cortex of rats after unilateral local cerebral infarction.更多还原