【摘要】 采用PCR-SSCP技术测定了4个黄羽肉鸡品种IGF-1基因2个SNP位点,并分析了它们对不同周龄体重及产蛋性状的遗传效应。结果显示,2个SNP位点分别发生了T→A、G→A的单碱基突变,各有3种基因型,京海黄鸡P1位点偏离Hardy-Weinberg平衡状态(P<0.01)。最小二乘方差分析表明,京海黄鸡、江村黄鸡AA型个体具有较高的300日龄产蛋量,与AB、BB型间差异显著(P<0.05),江村黄鸡AA型具有较高的12周龄和300日龄体重,石岐杂鸡AA型300日龄体重显著高于BB型(P<0.05),表明A为产蛋及体重增效基因;不同黄鸡品种均表现出CC型个体具有较高的300日龄体重和产蛋数(P>0.05)。更多还原

【Abstract】 Two SNP loci of IGF-1 gene were detected by PCR-SSCP method in Jinghai Yellow Chicken,Jiangcun Yellow hens,Shiqiza hens and Liyang hens,and their genetic effects on production performances were also analyzed.The results showed the mutations of T→A,G→A in P1,P2 loci were identified respectively,and there were three genotypes in each locus,three genotypes in P1 locus in Jinghai Yellow chicken were deviated the Hardy-Weinberg Equilibrium(P<0.05).The Least Square Means analysis showed,AA individuals in Jinghai Yellow Chicken,Jiangcun Yellow hens had more eggs than AB and BB ones in 300 days(P<0.05),and AA Jiangcun Yellow individuals had higher 12-week and 300-day weight,and AA Shiqiza individuals had higher 300-day weight than BB ones(P<0.05),and A was an egg-number and body weight enhancing gene,B was a leaky one.CC individuals in 4 breeds were all have higher 300-day weight and egg number(P>0.05).更多还原