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中国部分地区传染性法氏囊病病毒分子流病学调查

Investigation on Molecular Epidemiology of Infectious Bursal Disease in Parts of China

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【作者】 宇文延青高玉龙高宏雷祁小乐杨金雨王晓燕秦立廷林欢李俊山刘伟李铁强邓小芸王笑梅

【Author】 YUWEN Yanqing1,2,GAO Yulong1,GAO Honglei1,QI Xiaole1,YANG Jinyu1,WANG Xiaoyan1, QIN Liting1,LIN Huan1,LI Junshan1,LIU Wei1,LI Tieqiang1,DENG Xiaoyun1,WANG Xiaomei1(1.National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Heilongjiang,Harbin 150001; 2.Henan Normal University,Xinxiang,Henan 453007)

【机构】 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室河南师范大学

【摘要】 为调查我国鸡传染性法氏囊病病毒(IBDV)的流行情况,从我国11个省(市、自治区)采集法氏囊病料,分离鉴定鸡传染性法氏囊病病毒20株,运用RT-PCR方法对分离株的VP2基因进行扩增、测序和序列分析。研究表明,20株分离毒株中有19株具有IBDV超强毒株的分子特征,即222A、256I、294I和299S,与我国IBDV超强毒Gx株的核苷酸同源率在96.7%~99.4%,推导氨基酸同源率在98.2%~100%。遗传进化分析表明,所有分离毒株具有共同的祖先。

【Abstract】 In this study,complete VP2 genes of 20 IBDV isolats from 11 provinces (city or antomous region) were amplified using RT-PCR and sequenced to determine IBDV molecular epidemiology. The results showed that a majority (19/20) of the isolates had very virulent IBDV (vvIBDV)conserved amino acids:222A,242I,256I,294I and 299S,and only SH-h had P222. Compared to Chinese vvIBDV Gx strain,the homologous rates of nucleotide and amino atid were 97.5% to 99.4% and 98.9% to 100% ,respectively. Using serotype Ⅱ of IBDV as the outgroup, a phylogenetic tree based on VP2 nucleotide sequence was constructed. Our findings demonstrated that all isolates had a similar orgion with Gx strain and the other vvIBDV strains.

【基金】 国家肉鸡产业体系(nycytx-42-G3-01);973基金资助项目(2005CB523202)
  • 【文献出处】 中国家禽 ,China Poultry , 编辑部邮箱 ,2009年12期
  • 【分类号】S855
  • 【被引频次】17
  • 【下载频次】157
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