【摘要】 目的应用简便快速的核酸检测新方法——逆转录环介导等温扩增方法(Reverse Transcription Loop-mediated Isothermal Amplification,RT-LAMP)检测风疹病毒核酸,并与逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)方法进行比较。方法比较RT-LAMP方法与RT-PCR方法,检测吉林省风疹病毒分离株病毒核酸的检出率。结果对20份风疹标本分离病毒后,应用上述两种方法检测风疹病毒核酸,结果完全相同,阳性率均为55%(11/20),一致性达100%。结论两种方法检测结果相同,但RT-LAMP比RT-PCR更简便,快速。更多还原

【Abstract】 Objective To use a new simple Reverse Transcription Loop-mediated isothermal amplification(RTLAMP) method was applied to detect rubella virus nucleic acid and compared with Reverse TranscriptionPolymerase Chain Reaction (RT-PCR).Method Comparing the detection rate of the RT-LAMP method with that of RT-PCR for detecting rubella virus nucleic acid from rubella virus.Results The nucleic acid positive rates of all 11 strains of rubella virus were 100% by the two methods,the positive rate was 55%.Conclusion RT-LAMP is more simple and convenient than RT-PCR.更多还原