【摘要】 目的分析上海市2002年引起手足口病(Hand-foot-mouth Disease,HFMD)流行的柯萨奇病毒A组16型(Coxsackie virus A16,CVA16)的基因特征。方法对从上海市2002年HFMD患者标本中分离到的CVA16,采用逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)扩增VP1基因片段全长,并对扩增产物进行测序。根据VP1基因核苷酸序列与其它CVA16毒株序列(来源于基因库)构建亲缘性关系树,并且进行核苷酸和氨基酸同源性分析。结果从上海市2002年HFMD患者标本中共分离到7株CVA16,核苷酸和氨基酸的同源性分别为88.8%～99.7%和99.3%～100.0%,7株CVA16同属于B基因型,但分属不同分支。除了SH-18-CHN02毒株属于分支1外,其余6株CVA16毒株皆属于分支2。结论引起2002年上海市HFMD流行的CVA16毒株均为B基因型,但与中国大陆以往分离到的CVA16毒株存在差异,提示CVA16毒株在中国大陆已传播了较长时间,B基因型有多个不同分支的CVA16毒株传播链曾在中国大陆流行。因此应加强对2008年HFMD爆发中分离到的CVA16的分子流行病学监测,以阐明现阶段流行的CVA16基因型别和基因特征。更多还原

【Abstract】 Objective To reveal the genetic characteristic of Coxsackie virus A16 (CVA16) strains isolated during an epidemic of Hand-Foot-Mouth Disease (HFMD) in Shanghai in 2002. Methods CVA16 strains were isolated from the patients of HFMD during the epidemic in Shanghai in 2002. The VP1 coding gene of CVA16 strains was amplifi ed by reverse transcription-polymerase chain reaction (RT- PCR) and sequenced. The homology and phylogenetic analyses were done between the 7 isolates and other CVA16 strains downloaded from Genbank, based on the sequence of VP1 gene. Results 7 CVA16 strains were isolated from the patients of HFMD in Shanghai, which belonged to different lineages of genotype B. Among them, except for SH-18-CHN02 strain in lineage 1, other CVA16 strains were all in lineage 2. The homology of nucleotide and amino acid of 7 CVA16 strains were 88.8%~99.7% and 99.3%~100% respectively. Conclusions 7 CVA16 strains in this study were all genotype B. There were some variations compared with the other CVA16 strains isolated in China before. It suggested that CVA16 strains might have spread for a long time in China, which belonged to several different transmissions. To reveal the genotype and genetic characteristic of CVA16 prevalent in China, the surveillance of CVA16 should be strengthened to get more molecular epidemiologic data.更多还原