【摘要】 目的探讨热休克蛋白27(HSP27)、三叶因子Ⅱ(TFF2)在胃癌和癌前疾病中的表达及临床意义。方法应用免疫组化法检测经病理证实的正常胃黏膜组织20例、胃溃疡22例、慢性萎缩性胃炎24例和胃癌54例中HSP27和TFF2的表达情况,其表达水平以HSP27和TFF2的平均光定密度测定值表示,并对相关资料进行统计学分析。结果HSP27在正常胃黏膜中的表达光密度值(0.278±0.011)较胃溃疡(0.303±0.013)和慢性萎缩性胃炎(0.305±0.010)低,慢性萎缩性胃炎与正常胃黏膜组织中HSP27表达水平比较差异有统计学意义(P<0.05);HSP27在胃癌中的表达水平(0.318±0.022)较前三者高,差异均有统计学意义(P均<0.05);HSP27的表达与胃癌分化程度、淋巴转移有关(P均<0.05)。TFF2在胃溃疡(0.368±0.014)和慢性萎缩性胃炎(0.371±0.019)中的表达水平较正常胃黏膜(0.346±0.032)高,差异有统计学意义(P均<0.05)。胃癌中TFF2的表达水平(0.325±0.020)低于前3者,差异有统计学意义(P均<0.05);TFF2的表达与胃癌分化程度、浸润深度有关(P均<0.05)。结论HSP27和TFF2与肿瘤的发生密切相关,检测该指标可为胃癌诊断、判断预后和指导治疗提供依据。更多还原

【Abstract】 Objective To investigate the expressions of heat shock protein 27 (HSP27) and trefoil factor 2 (TFF2) in gastric cancer and gastric precancerous ailment and their possible clinical significance.Methods The expressions of HSP27 and TFF2 were studied by immunohistochemical SABC method in 120 gastric specimens including 20 normal groups, 22 gastric ulcers (GU), 24 chronic atrophic gastritis (CAG) and 54 gastric cancers (GC),and the results were shown as average light density assayed by image analysis software.Results The аverage light density of HSP27 in normal group was lower than those in GU and CAG,and the difference of аverage light density between CAG and normal group had statistical significance (0.305±0.010) vs (0.278±0.011),P<0.05.The аverаge light density of HSP27 in GC (0.318±0.022) was higher than those in other three groups (all P<0.05).The expression of HSP27 was significantly associated with the cellular differentiation and lymph node metastasis(all P<0.05).The аverage light of TFF2 in GU and CAG were higher than that in normal group(all P<0.05),while the expression of TFF2 in GC was lower than those in other three groups (all P<0.05).The expression of TFF2 was significantly associated with the cellular differentiation and the depth of tumor invasion(all P<0.05).Conclusions HSP27 and TFF2 may play an important role in carcinogenesis and progression of gastric carcinoma. The detections of HSP27 and TFF2 can provide basis for diagnosis,evaluating prognosis and conducting cure of gastric cancer.更多还原