【摘要】 目的探讨p16、Ki-67联合应用在宫颈上皮内瘤变(CIN)诊断及分级中的意义及人乳头状瘤病毒(HPV)感染与CIN的关系。方法分别对160例宫颈各类病变采用免疫组化方法检测p16、Ki-67蛋白表达情况,并行人乳头状瘤病毒分型检测。结果①宫颈上皮内低度瘤变(CINⅠ)中,p16阳性率为85.7%,Ki-67阳性率为67.8%,较宫颈良性病变的阳性率明显增加(P<0.01);宫颈上皮内高度瘤变(CINⅡ-Ⅲ)中,p16阳性率为100%,Ki-67阳性率为98.3%,两者相比差异极显著(P<0.01)。在CINⅡ-Ⅲ中,p16、Ki-67表达呈正相关。②高危型HPV在宫颈良性病变、CINⅠ和CINⅡ-Ⅲ病变组感染者分别为30.6%、75.7%和93.3%,宫颈CINI和CINⅡ-Ⅲ明显高于良性病变组,差异极显著(P<0.01)。随着宫颈上皮内瘤变程度加重,HPV感染与p16表达呈正相关。结论p16、Ki-67联合应用可作为宫颈良性病变与CINⅠ以及CINⅠ与CINⅡ-Ⅲ鉴别的重要标记物;高危型HPV感染与宫颈上皮内瘤变密切相关,在高危人员筛查与判断预后上有重要意义。更多还原

【Abstract】 Objective To investigate the expression of p16 and Ki-67 and detection of high-risk HPV in the diagnosis and grading of cervical intraepithelial neoplasia(CIN). Method Immunohistochemical staining was used for detection of p16 and Ki-67 protein expression in 160 cases of various cervical lesions.Quick nuclear hybridization method was used for detection of high-risk HPV.The data were analysed by SPSS software.Result(1) In CINⅠ,p16 and Ki-67 expression rates were 85.7% and 67.8% respectively,which were significantly higher than that of benign cervical lesions(P<0.001).In CINⅡ-Ⅲ,p16 and Ki-67 expression rates were 100% and 98.3% respectively,which were significantly higher than that of CINⅠ(P<0.001).In addition,p16 expression was strongly associated with Ki-67 expression in CINⅡ-Ⅲ.(2) High-risk HPV infected rate was 75.7% and 93.3% in CINⅠand CINⅡ-Ⅲ,respectively, which is significantly higher than that of benign cervical lesions(30.6% P<0.01).In addition,high risk HPV infected rate was strongly associated with expression of p16 protein.Conclusions p16 and Ki-67 can be used as important markers to distinguish CINⅠfrom benign cervix lesions as well as CINⅠfrom CINⅡ-Ⅲ.High-risk HPV infection is closely related with CIN,and thus detection of high-risk HPV is of great importance in screening high risk group and evaluating the prognosis of cervical diseases.更多还原