【摘要】 目的:观察从纳豆杆菌中获得纳豆激酶对急性血瘀模型大鼠血液流变学及血小板聚集的影响。方法:以肾上腺素附加冰浴制备大鼠急性血瘀模型,观察纳豆激酶对大鼠血液流变学的影响;分别采用ADP、凝血酶和花生四烯酸诱导大鼠血小板聚集,用比浊法测定不同时间点的聚集率及最大聚集率。结果:纳豆激酶能明显降低血瘀大鼠的全血低、中、高切黏度,血浆黏度,红细胞压积,纤维蛋白原,红细胞聚集指数及电泳指数;明显抑制ADP、凝血酶、花生四烯酸诱导的血小板聚集,其ED50分别为1 431.9 IU/kg、1 055.3 IU/kg、1 390.8 IU/kg。结论:纳豆激酶能明显改善血瘀大鼠的血液流变学指标,具有显著的抗血小板聚集作用。更多还原

【Abstract】 AIM: To observe the effects of nattokinase from bacillas natto on hemorheology and platelet aggregation in rats with acute blood stasis model. METHODS: A model of blood stasis was established by means of giving rats an injection of adrenalin and making it swim in ice-cold water,the change of hemorheology was observed after the rats took nattokinase for 10 d.Rat platelet aggregation induced by ADP,thrombin,and arachidonic acid,and maximum aggregation were measured by turbidimetric method at different time. RESULTS: Nattokinase could markedly decrease the whole blood viscosity with low,middle,high shear rate,plasma viscosity,hematocrit,fibrinogen, erythrocyte aggregation index and electrophoresis index.Nattokinase significantly inhibited platelet aggregation induced by ADP,thrombin and arachidonic acid,and the values of ED₅₀（median effective dose） were（1 431.9 IU/kg,） 1 055.3 IU/kg and 1 390.8 IU/kg. CONCLUSION: Nattokinase could markly improve hemorheology in rats with acute blood stasis,and inhibit the platelet aggregation.更多还原