【摘要】 目的探讨原发性肝癌患者的癌细胞、外周血白细胞、淋巴细胞和粒细胞等系列化疗药敏或耐药的关系及可行性。方法采用MTT法分别进行体外药敏或耐药试验,检测31例原发性肝癌患者新鲜肿瘤组织标本的癌细胞和外周血有核细胞中的白细胞、淋巴细胞及粒细胞对10种化疗药物的敏感性或耐药性。结果除HCPT(羟基喜树碱)外,癌细胞对其它9种化疗药物的抑制率均明显高于白细胞、淋巴细胞和粒细胞的抑制率;癌细胞、白细胞和淋巴细胞对前5个由高到低化疗药物的敏感性大致相同,癌细胞分别与白细胞、淋巴细胞及白细胞与淋巴细胞的抑制率呈正相关关系(P<0.01、0.05)。结论对无法取得组织标本确定药敏结果的原发性肝癌患者以外周血白细胞、淋巴细胞药敏或耐药试验的检测是可行的,并能为临床扩大优化个体治疗方案提供实用而有参考价值的实验依据。更多还原

【Abstract】 Objective To investigate the relationship between serial drug sensitivity or resistance to liver cancer cells and peripheral leucocytes,lymphocytes and granulocytes.Methods The sensitivity or resistance of 10 kinds of anticarcinogen for peripheral leucocytes,lymphocytes,granulocytes and the tumor cells from 31 cases of liver cancer were tested by the MTT assay. Results Except HCPT,the inhibition rates of the tested drugs in the cancer cells were apparently higher than that in the leucocytes, lymphocytes and granulocytes.The sensitivity of the top 5 anticarcinogen in cancer cells,leucocytes and lymphocytes were approximately.The correlativity between the inhibition rates of arbitrary two sets cells were all significant(P<0.01). Conclusion It was feasible to detect the sensitivity or resistance of peripheral leucocytes and lymphocytes of liver cancer patients. It could provid practical and valuable reference to optimize the individual treatment in liver cancer chemotherapy.更多还原