【摘要】 本研究对常见枫斗类石斛cpDNA的psbA-trnH区进行了测序,利用MEGA4.0对该区序列进行了比较分析。结果显示:15种常见的枫斗类石斛的psbA-trnH序列长度为721～767bp,变异位点42个,简约信息位点11个。以Kimura-2参数计算遗传距离,常见枫斗类石斛的种间遗传距离为0.0013～0.0183,平均遗传距离为0.0148。不同石斛种间均存在序列差异,全序列中共出现6处indels,导致种间片段长度差异较大;暂未发现所检测的枫斗类石斛居群间在psbA-trnH区的序列存在差异。利用枫斗类石斛psbA-trnH序列数据库及遗传分析软件,通过对待检种的psbA-trnH序列的测定,成功鉴别了待检种。cpDNA的psbA-trnH区可作为枫斗类石斛分子鉴别的候选标记。更多还原

【Abstract】 The psbA-trnH regions of Dendrobium species of Fengdous were sequenced by our research group.The psbA-trnH sequences of fifteen Dendrobium species were analyzed with software MEGA 4.0.The results showed that the lengths of sequences varied from 721 to 767 bp.The variable sites were 42 while the informative sites were 11.Genetic distances were calculated using the Kimura 2-parameter model.Genetic distances varied from 0.001 3 - 0.018 3 among fifteen species while the average genetic distance was 0.014 8.The interspecies differences of psbA-trnH regions were demonstrated.Six indels happened in this fragment,which led to the great difference of sequence lengths among fifteen species.We found that there were no population differences in the psbA-trnH region of various species of Fengdous so far.By using the database of various Dendrobium species of Fengdous and two genetics software,the botanical origin of the inspected species of Fengdous was authenticated successfully by sequencing the psbA-trnH regions.The psbA-trnH region of cpDNA can be used as a candidate marker for authentication of Dendrobium species of Fengdous.更多还原