【摘要】 目的:建立同时测定血浆中阿糖胞苷和阿糖尿苷浓度的高效液相色谱法,并用于1例急性非淋巴细胞白血病患儿的血药浓度监测。方法:采用反相高效液相色谱法,色谱柱:XTerra C18(4.6 mm×250 mm,5μm);流动相:0.01 mo.lL-1的磷酸盐缓冲液(pH=6.0);检测波长:280 nm;流速:0.95 mL.min-1;柱温:30℃。结果:阿糖胞苷血药浓度在0.25~21.74 mg.L-1范围内线性关系良好(r=0.999 6),检测限为0.1 mg.L-1,日内、日间RSD小于5.0%和8.0%;阿糖尿苷血药浓度在0.99~86.96 mg.L-1范围内线性关系良好(r=0.999 3),检测限为0.4 mg.L-1,日内、日间RSD小于5.0%和8.0%。阿糖胞苷及阿糖尿苷平均提取回收率高于96.0%。结论:本方法简单、快速、准确,适用于临床上阿糖胞苷和阿糖尿苷的血药浓度监测及药动学研究。更多还原

【Abstract】 Objective: To establish a method for the determination of cytarabine(Ara-C) and uracil arabinoside(Ara-U) in plasma simultaneously by high liquid performance chromatography,and apply it to an acute non-lymphocytic leukemia children.Methods: The assaying was performed on a 4.6 mm×250 mm,5 μm particle,XTerra C18 column,the mobile phase consisted of 0.01 mol·L-1 phosphate buffer(pH =6.0).The flow rate was 0.95 mL·min-1,the detection wavelength of UV was 280 nm,and column temperature was 30℃.Results: The linear blood concentration range for Ara-C was0.25 ~ 21.74 mg·L-1(r=0.999 6)with the minimum detection at 0.1 mg·L-1,the relative standard deviations of intra-day and inter-day were below 5.0% and 8.0%.The linear blood concentration range for Ara-U was 0.99~86.96 mg·L-1(r=0.999 3)with the minimum detection at 0.4 mg·L-1,the relative standard deviations of intra-day and inter-day were below 5.0% and 8.0%.The average recovery of Ara-C and Ara-U was more than 96.0%.Conclusions: The method is simple,fast,accurate and clinically applicable to Ara-C and Ara-U plasma concentration monitoring and pharmacokinetic studies.更多还原