【摘要】 目的探讨肥大细胞、巨噬细胞、转化生长因子β1在IgA肾病患者肾组织中的表达与肾组织病变严重程度的关系。方法收集49例IgA肾病患者的临床资料和肾活检标本,采用免疫组织化学技术检测肥大细胞、巨噬细胞、转化生长因子β1在IgA肾病患者肾组织中的表达,并分析其意义。结果正常肾组织中偶见或无肥大细胞、巨噬细胞及转化生长因子β1的存在,而在IgA肾病患者肾组织中肥大细胞、巨噬细胞、转化生长因子β1表达明显增强(肥大细胞检出阳性率为43%,巨噬细胞检出阳性率为41%,转化生长因子β1检出阳性率为51%);并且肥大细胞、巨噬细胞、转化生长因子β1的表达与IgA肾病肾组织病变程度呈正相关关系(r=0.9901,0.9897,0.9926,P均<0.05)。结论肥大细胞可能是IgA肾病肾间质纤维化的重要参与者;巨噬细胞可能是IgA肾病发生发展过程的重要参与者;转化生长因子β1可能是肥大细胞、巨噬细胞的重要趋化因子。更多还原

【Abstract】 Objective To investigate the profiles of mast cells,macrophages and transforming growth factor β1 in renal tissues of patients with IgA nephropathy and their correlation with renal lesions.Methods Renal biopsy specimens and clinical data were collected from 49 patients with IgA nephropathy.The profiles of mast cells,macrophages,transforming growth factor β1 in renal tissues were detected using immunohistochemistry and analyzed for their significance.Results Mast cells,macrophages and transforming growth factor β1 were absent or appeared occa-sional in normal tissues but found in substantial amouts in IgA nephropathy,with mast cells detected in 43%,macrophages in 41% and transforming growth factor β1 in 51% of the patients.The profiles of mast cells,macrophages,TGF-β1 expression were positively correlated with the lesions of renal tissue(rs=0.9897,0.9910,0.9926,all P<0.05).Conclusion In IgA nephropathy,the roles of mast cells as involved in renal interstitial fibrosis,macrophages as in development and progression of disease,and transforming growth factor β1 as an important chemokine may be suggested.更多还原