【摘要】 目的研究缺氧诱导因子1α(HIF-1α)和碱性成纤维生长因子受体(BFGFR)在结直肠腺癌中的表达和相互作用,以及它们与结直肠腺癌生物学行为的关系。方法采用免疫组化SP法检测了60例手术切除的结直肠腺癌及癌旁组织、20例正常大肠黏膜组织中的HIF-1α和BFGFR表达。结果HIF-1α和BFGFR在结直肠腺癌中的表达阳性率分别为61.7%和58.3%,明显高于癌旁组织的10.0%和11.7%(P<0.05)。正常肠黏膜中未检出HIF-1α、BFGFR。HIF-1α和BFGFR在结直肠腺癌中表达与性别、年龄、肿瘤大小、肿瘤位置和分化程度无关(P>0.05),但与肿瘤Dukes分期相关(P<0.05)。结直肠腺癌组织中HIF-1α与BFGFR表达具有相关性(P<0.05)。结论结直肠腺癌组织中的HIF-1α和BFGFR表达水平增高,参与了结直肠腺癌的发生发展过程;且HIF-1α与BFGFR可能起到协同作用,共同促进肿瘤的发生。更多还原

【Abstract】 Objective To study the expression and interaction of hypoxia-inducible factor-1α(HIF-1α) and basic fibroblast growth factor receptor (BFGFR) in colorectal adenocarcinoma. Methods The expressions of HIF-1α and BFGFR in intestinal biopsy specimens taken from 60 patients with colorectal adenocarcinoma and 20 subjects with irritable bowel syndrome were detected by immunohistochemical staining(SP method). Results Positive rates of HIF-1α and BFGFR in colorectal adenocarcinoma tissues were 61.7% and 58.3%,respectively,which were significantly higher than 10.0% and 11.7% in the normal intestinal mucosal tissues (P<0.05). There were no correlations of the expressions of HIF-1α and BFGFR with gender,age,tumor size,tumor position or cell differentiation (P>0.05),but were significantly correlated with Dukes staging(P<0.05). The HIF-1α expression level was correlated with BFGFR in colorectal adenocarcinoma tissues. ConclusionOverexpression of HIF-1α and BFGFR in colorectal adenocarcinoma tissues may synergistically participate in the carcinogenesis and development.更多还原