【摘要】 目的探讨Survivin蛋白在肺腺癌所致恶性胸腔积液中的表达及其临床意义。方法采用免疫细胞化学和蛋白印迹技术检测49例恶性胸腔积液和25例良性胸腔积液中Survivin蛋白的表达,同时送检胸液脱落细胞学作为对照,分析Survivin与肺腺癌所致恶性胸腔积液的临床特征和疗效之间的关系。结果Survivin在恶性胸腔积液中的阳性表达率为81.6%(40/49),明显高于胸液细胞学检查的阳性率57.1%(28/49),二者比较差异有显著性(P<0.05),联合检测阳性率达87.7%;同时也显著高于良性组8.00%(2/25)(P<0.05)。Survivin的表达在患者性别、年龄、吸烟、有无淋巴结转移和远处转移中差异无显著性;在恶性组中,胸水Survivin蛋白表达阳性患者治疗有效率为52.5%(21/40),明显低于阴性患者的88.8%(8/9)(P<0.05);但细胞质和细胞核之间差异无显著性(P>0.05)。结论肺腺癌所致恶性胸腔积液中Survivin蛋白的表达明显增高,检测胸水Survivin的表达可作为恶性胸腔积液早期诊断的一个新的生物标记物,对鉴别良恶性胸腔积液和预测恶性胸腔积液疗效有一定的参考价值。更多还原

【Abstract】 Objective To investigate the expression of survivin protein in the malignant pleural effusions with lung adenocarcinoma and its clinical significance.Methods The expression of survivin protein was detected by immunocytochemistry and western blot in 49 malignant pleural effusions with lung adenocarcinoma and 25 benign pleural effusions; the determination of exfoliated cytology in malignant pleural effusions,was also set as a control,and analyzed the relationship between the survivin protein expression and clinical characteristic,therapeutic effect in patients with malignant pleural effusions.Results Survivin protein expression rates in the malignant pleural effusions were 81.6% (40/49),significantly higher than that of pleural effusion cytology (52.1%,28/49,P<0.05) and that in benign group (8.00%,2/25,P<0.05). The positive rate of combined both of above methods was 87.7% (43/49).At the same time,there were no relationship between their age,sex,smoking,and lymph node metastasis or distant metastasis in malignant pleural fluid survivin expression. In malignant group,the therapeutic effect rate in patients with survivin positive expression was 52.5% (21/40),significantly lower than that in negative group (88.8%,8/9,P=0.045),while there is no difference between the cell nucleus and cytoplasm (P=0.119).Conclusion Over-expression of survivin protein is found in malignant pleural effusions with lung adenocarcinoma.The determination of survivin expression in pleural effusions may be a new earlier biomarker in diagnosis of malignant pleural effusions,which there is an important referential value in making a difference between malignant and benign pleural effusions,and in assessing therapeutic effect of malignant pleural effusions.更多还原