【摘要】 背景:前期体外实验已经证明神经生长因子乙交酯-丙交酯共聚物与神经生长因子复合支架具有良好的细胞黏附亲和性;同时观察到其随时间推移可稳定释放神经生长因子。目的:将生物可降解材料乙交酯-丙交酯共聚物与神经生长因子复合支架移植入大鼠脊髓半横断损伤动物模型中,观察其对脊髓损伤髓鞘再生的影响。设计、时间及地点:随机对照动物实验,于2006-05/07在首都医科大学附属北京市神经外科研究所损伤修复实验室完成。材料:乙交酯-丙交酯共聚物与神经生长因子复合支架材料,其中0.02g乙交酯-丙交酯共聚物中含有1μg神经生长因子。方法:45只成年Wistar大鼠制备T8~9脊髓半横断损伤模型,随机分为单纯支架组20只和复合支架组25只,分别移植乙交酯-丙交酯共聚物支架和掺有神经生长因子的乙交酯-丙交酯共聚物支架。主要观察指标:于术后1,4,8和12周进行髓鞘碱性蛋白免疫组织化学及超微结构检测观察髓鞘再生的情况。结果:复合支架组苏木精-伊红染色观察脊髓损伤程度较单纯支架组明显减轻。复合支架组髓鞘碱性蛋白免疫组织化学阳性颗粒明显多于单纯支架组。透射电镜观察复合支架组大鼠新生髓鞘明显多于单纯支架组。结论:乙交酯-丙交酯共聚物联合神经生长因子促进髓鞘再生的效果优于单纯乙交酯-丙交酯共聚物。更多还原

【Abstract】 BACKGROUND: A previous in vitro experiment has shown that there was a good adherent compatibility between poly(lactide-co-glycolide acid) (PLGA) and nerve growth factor (NGF); in addition, the PLGA/NGF composite scaffold could stably release NGF with the time prolongation. OBJECTIVE: To observe the effect of PLGA/NGF composite scaffold implantation on remyelination in a rat model of spinal cord injury. DESIGN, TIME AND SETTING: A randomized controlled animal experiment was performed at Laboratory of Injury Repair, Beijing Institute of Neurosurgery, Capital Medical University between May and July 2006. MATERIALS: PLGA/NGF composite scaffold (every 0.02 PLGA containing 1 μg NGF) was used in this experiment. METHODS: A total of 45 adult Wistar rats were chosen to prepare T8-9 hemi-transected spinal cord injury models. All rats were randomly divided into PLGA alone group (n=20) and composite scaffold group (n=25), separately implanting the PLGA scaffold and the PLGA/NGF composite scaffold. MAIN OUTCOME MEASURES: Remyelination was detected using myelin basic protein immunohistochemistry and ultrastructure methods at week 1, 4, 8, and 12 postoperatively. RESULTS: Hematoxylin-eosin staining demonstrated that spinal cord injury in the composite scaffold group was lighter than PLGA alone group. Myelin basic protein immunohistochemistry indicated that number of positive particles in the composite scaffold group was greater than PLGA alone group. Transmission electron microscopy showed that number of proliferating myelin sheath in the composite scaffold group was greater than PLGA alone group CONCLUSION: Effect of PLGA/NGF composite scaffold on remyelination is superior to that of PLGA alone.更多还原