【摘要】 目的探讨艾滋病病毒1型B′亚型(HI V-1 B′)慢性感染人群连续两年血浆中和能力的变化,及其与疾病进展的关系。方法通过对两年前后127例HI V-1 B′亚型感染者进行CD4和病毒载量(VL)的检测,采用基于TZM-b1细胞的中和实验方法,检测感染者血浆分别针对HI V-1临床分离株1597和实验室适应株SF33两种病毒的中和能力。通过分析中和50%病毒进入细胞的血浆浓度(EC50)与CD4计数和VL的相关性,阐明血浆中和水平与疾病进展的关系。结果2005年和2007年CD4分别为(454±131)个/μl和(440±182)个/μl(t=1.02,P=0.309),VL(log10)分别为(4.03±0.89)拷贝/ml和(4.21±0.89)拷贝/ml(t=2.72,P=0.008);血浆对1 597株有中和活性的分别有91例(71.65)和75例(66.93%),其EC50分别为(65.15±0.51)和(55.59±0.59)(t=1.16,P=0.248);对SF33株有中和活性的分别有108例(85.04%)和101例(85.04%),其EC50为(205.65±0.52)和(139±0.47)(t=3.57,P<0.001)。聚类分析显示,EC50与VL的关联性较强,但EC50的变化与VL的变化的相关性没有统计学意义(SF33∶r=0.07,P=0.41;1597∶r=0.12,P=0.18)。结论从2005年到2007年,慢性HI V-1B′亚型感染者的VL显著升高,尽管对病毒的中和水平降低,但血浆中和水平的降低与VL的升高没有直接的相关性。更多还原

【Abstract】 Objective To study association of disease progression with neutralizing activity of plasma among 127 chronic HIV-1B′ infected former blood donors in Anhui province.Mechods The viral load(VL)and CD4 count were tested in 2005 and 2007 respectively,and the neutralizing activity of plasma against primary virus strain(1597)and lab-adapted strain(SF33)was also detected by using Luciferase assay system based on TZM-bl cell line.The plasma concentration of inhibiting 50% of viruses to enter cells was expressed as EC50.The relation between EC50 and CD4 count or VL was analyzed respectively.Results The CD4 count was 454±131 in 2005 and 440±182 in 2007(t=1.02,P=0.309),while the VL(log10)was 4.03c/ml±0.89 in 2005 and 4.21c/ml±0.89 in 2007(t=.272,P=0.008).Out of the 127 cases under study,91(71.65%)and 75(66.93%)in their plasma had neutralizing activity against 1597 in 2005 and 2007,respectively while 108(85.04%)and 101 in their plasma had neutralizing activity against SF 33 in the two respective years.EC50 against SF33 fell down from 205.65±0.52 in 2005 to 139±0.47 in 2007(t=3.56,P<0.001),but EC50 against 1597 fell down only slightly from 65.15±0.51 in 2005 to 55.59±0.59 in 2007(t=1.16,P=0.248).Correlation analysis showed no significant correlation between falling EC50 and VL(SF33∶r=0.07,P=0.41;1597∶r=0.12,P=0.18),although neutralizing activity had closer relationship with VL analyzed by the varcluster analysis.Conclusion From 2005 to 2007,plasma VL of the 127 chronic HIV-1B′ infected individuals showed significant rise,while the neutralizing activity against SF33 fell down.However,there was no statistical correlation between the decreasing EC50 and increasing VL.更多还原