【摘要】 采用分子生物学方法对宁波北仑港冬季水体中的浮游细菌多样性进行了研究.提取水样中细菌基因组总DNA,以细菌16S rDNA通用引物进行PCR扩增,扩增产物经分子克隆、测序与序列分析,对水样中的细菌建立了16S rDNA克隆文库和系统发生树.结果表明,浮游细菌群落具有较高的多样性,34个克隆子分属2个不同的细菌类群,6个克隆子属于未知类群,优势细菌类群为Pro-teobacteria类群(变形菌类群),占80%;细菌优势类群顺序为β-Proteobacteria类群(32.5%)、γ-Proteobacteria类群(25%)、α-Proteobacteria类群(15%)、ε-proteobacteria类群(7.5%)、Firmicutes类群(厚壁菌类群)(5%).这一结果与近年来国内外有关港口微生物多样性的报道较为一致.用DNAStar中Clustalw程序从测序的40个克隆子中选出17个OTU进行系统发育分析,同样表明浮游细菌多样性较强.更多还原

【Abstract】 The bacterioplankton diversity in waters of Beilun harbor in November,2007,was studied using techniques of molecular biology.The clone library of 16S rDNA and the phylogenetic tree were constructed with extraction of bacterial DNA,PCR amplification of bacterial 16S rDNA by universal primers,molecular clone,sequencing of 16S rDNA fragments and sequence analysis.The results indicated that the bacterial diversity was high in that 34 belonged to 2 different published phyla and 6 belonged to unknown phylum.The dominant bacterial community was Proteobacteria,which accounted for 80% of the total.The bacterial community succession were listed as follows,β-Proteobacteria(32.5%),γ-Proteobacteria(25%),α-Proteobacteria(15%),ε-proteobacteria(7.5%) and Firmicutes(5%).The result was much consistent compared with the microorganism diversity data published home and abroad in recent years.The phylogenetic results demonstrated higher bacterial diversity after 17 OTU phylotypes from 40 sequenced clones were selected and analyzed with Clustalw of DNAStar Software.更多还原