【摘要】 目的探讨WASP家族Verprolin同源蛋白-1(WAVE-1)测定在急性白血病患者中的临床意义。方法运用逆转录-聚合酶链反应(RT-PCR)和Western blotting法分别测定65例急性白血病、21例非恶性血液病及50例正常对照者血清中WAVE-1含量,并进行比较。结果急性白血病患者血清WAVE-1含量明显高于正常对照组(P<0.01);非恶性血液病患者血清WAVE-1低于急性白血病患者(P<0.01),而与正常对照组比较无显著性差异(P>0.05)。化疗后完全缓解的急性白血病患者血清WAVE-1含量明显低于化疗前(P<0.01),复发者血清WAVE-1含量又复升高(P<0.01)。结论检测WAVE-1对急性白血病诊断、鉴别诊断及预后判断均有参考价值。更多还原

【Abstract】 Objective To assess the clinical significance of detection of WASP-family verprolin-homologous protein 1(WAVE-1) protein in patients with acute leukemia(AL).Methods The expression of WAVE-1 in 65 AL patients,21 patients with non-malignant hematologic disease(NMHD) and 50 normal controls was detected by RT-PCR and Western blotting.Results The WAVE-1 levels in AL patients were significantly higher than normal controls(P<0.01);while in patients with NMHD,the WAVE-1 was lower than the AL(P<0.01),but no difference as compared with the controls(P>0.05).For AL patients with complete remission after chemotherapy,the WAVE-1 level was lower than before(P<0.01).Conclusion The determination of serum WAVE-1 levels is of value for reference in the diagnosis,differential diagnosis and predicting the prognosis of acute leukemia.更多还原