【摘要】 目的探讨细胞因子PDGF对瘢痕成纤维细胞生物学行为的影响、病理改变,及其对瘢痕的作用机制,为临床预防和治疗瘢痕提供分子及病理学水平的理论依据。方法对大鼠动物创伤模型进行体内实验,采用倒置显微镜、HE染色、Ca son氏改良三色染色、Hamason-Lush-bangh氏染色法、透射电镜等方法进行观察、分析,以研究细胞因子PDGF对瘢痕成纤维细胞生物学行为的影响以及病理改变。结果大鼠动物创伤模型病理染色与透射电镜观察显示,与对照组相比,实验组局部注射细胞因子PDGF后,促进了成纤维细胞的生长增殖,刺激了胶原纤维的合成、增生和沉积。结论细胞因子PDGF对成纤维细胞的生长趋势与增殖活性有明显的促进作用,刺激了胶原纤维的合成、增生和沉积。更多还原

【Abstract】 Objective To explore the mechanism of cytokines for the scars,we study effects PDGF on the fibroblasts of scars in biological behavior and pathological change.It can provide the basic of theory in molecular biology and pathobiology.Materials and Methods Wounds were created on a Sprague-Dawley(SD) rat model.The results were observed and analysed by light inverted microscope(LM),transmission elextron microscope(TEM),HE staining,Cason’s staining and flow cytometry(FCM).It was determined that effects of PDGF on scars in biological behavior and pathological change.Results In vivo(rat model):using pathological staining and TEM,compared to the controls,it could be obtained that the fibroblasts,proliferation was enhanced and collagen synthesis was increased by topically applying PDGF.Conclusions PDGF can increase fibroblasts proliferation and collagen synthesis,secretion and accumulation.更多还原