【摘要】 采用克雷伯氏菌(Klebsiella pneum oniaeC ICC 10011)发酵生产2,3-丁二醇,并对2,3-丁二醇的盐析分离工艺进行了考察。通过实验确定了以葡萄糖为底物微氧批式流加发酵的条件,发酵液中2,3-丁二醇和3-羟基丁酮的质量浓度分别为90.98 g/L和12.40 g/L,2,3-丁二醇的摩尔转化率为82.7%,生产强度达到2.1 g/(L.h)。对发酵液中2,3-丁二醇的盐析分离研究表明,K2HPO4和K3PO4对2,3-丁二醇的盐析效果优于K2CO3。当发酵液浓缩70%后,加入质量分数为45%的K2HPO4,2,3-丁二醇的分配系数达到9.10,回收率为79.37%;上相中2,3-丁二醇的质量浓度达到420 g/L;此时3-羟基丁酮的分配系数和回收率分别为11.9和83.48%。更多还原

【Abstract】 The bioconversion of 2,3-butanediol by Klebsiella pneumoniae CICC 10011 was studied with a medium based on glucose in fed-batch fermentations,and the salting out separation was explored.The final concentration of 2,3-butanediol and acetoin in the fermentation broth reached 90.98 g/L and 12.40 g/L,respectively.The molar yield of butanediol was 82.7% and the productivity was 2.1 g/(L·h).The effects of concentration cycles and salt concentrations on the extraction of 2,3-butanediol in flocculated broth were also studied.When the fermentation broth was concentrated to 70%,45% K2HPO4 was added,the partition coefficients and the recovery rate of 2,3-butanediol and acetoin reached 9.10,and 79.37%,respectively.The concentration of 2,3-butanediol in top phase reached 420 g/L,the partition coefficient and the recovery rate reached 11.9 and 83.48%.Results indicate that the process has advantages of simple,energy saving,and good potential for application.更多还原