【摘要】 为了开发新型天然抗菌物质,本研究以猪粪便中分离出的一株产抗猪链球菌细菌素产生菌屎肠球菌LPL420P06为试材,对其产生的细菌素进行了理化特性研究。结果表明,该细菌素具有良好的热稳定性,在90℃、30min热处理的条件下仍能保持90%以上的活性;易被胰蛋白酶、蛋白酶K、木瓜蛋白酶、中性蛋白酶失活,表明该细菌素是一类蛋白类物质;在pH5～9范围内37℃、4h处理,活性保持稳定;抑菌谱实验结果表明,该细菌素不仅对单增李斯特氏菌、金黄色葡萄球菌、猪链球菌等多种革兰氏阳性病原菌有明显的抑制作用,而且对沙门氏菌、大肠杆菌等部分革兰氏阴性菌有不同程度的抑制作用,显示出该细菌素具有很好的开发应用前景。更多还原

【Abstract】 In this study, the biological characteristics of bacteriocin produced by Enterococcus faecium LPL420P06 isolated from fresh feces of pigs that can inhibit Streptococcus suis were investigated. The experimental results revealed that the bacteriocin has good thermal stability and it can remain 90% of activity when heated at 90 ℃ for 30 min. It is sensitive to proteolytic enzymes such as trypsin , proteinase K, papain and neutral proteinase, indicating that the bacteriocin is a kind of protein. It is still active after incubation in pH 5 to 9 environment for 4 h at 37 ℃. The tests on antimicrobial spectrum suggested that it not only exhibits obvious inhibition against gram-positive, such as Listeria bacteria, Staphylococcus aureus and Streptococcus suis etc, but also can inhibit several gram-negative bacteria such as Salmonella and Escherichia coli. All these show that it has a good prospect of development and ultilization.更多还原