【摘要】 本实验以牛骨为原料,提供了一种经过粉碎、脱脂、脱钙、酶解、盐析、纯化、冷冻干燥等步骤来制备天然牛骨胶原的方法。并采用SDS-PAGE凝胶电泳、紫外吸收光谱、红外吸收光谱、氨基酸含量分析方法,对其进行了结构表征。结果表明,纯化的天然牛骨胶原的纯度达到99%,属于胶原I型([α1(I)]2α2(I)),且完整地保持了胶原的三螺旋结构,两条肽链的相对分子量为α1=123kD,α2=112kD。更多还原

【Abstract】 In this study, high-purity bone collagen was prepared from bovine bone via smashing, degreasing, decalcifying, hydrolyzing with enzyme, salting out, purifying and freeze-drying, and then its characterization was analyzed by SDS-PAGE electrophoresis, ultraviolet absorption spectrum, infrared absorption spectrum and amino acid component analysis. The results showed that the purity of the collagen product reaches 99%; it belongs to type I collagen ([α1(I)]2α2(I)) with integrated three helical structure, and the related molecular weights of its peptide chainsα1 andα2 are 123 kDa and 112 kDa, respectively.更多还原