【摘要】 采用高效液相色谱(HPLC)法,建立了石硖龙眼的HPLC指纹图谱。色谱条件为:Nucleosil C18反相柱(250mm×4mm,5μm),流动相为0.01mol/L的KH2PO4和甲醇,二元梯度洗脱,流速0.8ml/min,检测波长260nm,柱温4℃。结果表明:14批次的石硖龙眼水溶性提取物色谱图共有峰13个。经"中药指纹图谱鉴别分析系统"统计分析,共有峰相对保留时间的相对标准偏差在1.2%以下,相似度均在0.967以上。符合指纹图谱的要求,可建立共有模式,作为科学评价石硖龙眼产品质量的检测方法。更多还原

【Abstract】 In this study,HPLC analysis on Shixia longan(Dimocarpus longan Lour.cv.Shixia) was performed on reversed-phase Nucleosil C18 column(250 mm × 4.0 mm,5 μm) by using mobile phase 0.01 mol/L KH2PO4 and CH3OH,dual gradient elution was proceeded with the flow rate of 0.8 ml/min,and operation conditions were set as follows:detection at 260 nm and column temperature 40 ℃.Results showed that there are 13 common peaks in HPLC chromatogram of water-soluble compo-nents of 14 Shixia longan samples collected from different areas.Moreover,by calculated with fingerprint analysis system of Chinese medicine,relative standard deviations(RSD) of relative retained time of common peaks are below 1.2% and their similarity is above 0.967.Therefore,the established chromatographic fingerprint is proved to be a scientific quality evaluation method for Shixia longan.更多还原