【摘要】 研究茯苓皮中三萜类成分的体外抑菌活性。采用香草醛-冰乙酸-高氯酸显色法测定茯苓皮中的三萜含量;采用生长速率法对茯苓皮中三萜的体外抑菌活性进行研究。茯苓皮甲醇提取液的化学显色反应结果均为阳性,表明提取液中含有三萜类物质。茯苓皮中三萜的百分含量为1.58%。茯苓皮三萜提取液对大肠杆菌、金黄色葡萄球菌、绿脓杆菌的最低抑菌浓度分别为30%、30%、60%,即47、47、95μg/ml。茯苓皮三萜对大肠杆菌、金黄色葡萄球菌的抑菌效果受时间因素影响很小,抑菌效果稳定:而对绿脓杆菌的抑菌效果受时间因素影响较大,稳定性较差。茯苓皮三萜对大肠杆菌、金黄色葡萄球菌、绿脓杆菌都有较好的抑制作用,可作为治疗化脓性感染药物及祛痘活性的天然护肤品加以研究开发。更多还原

【Abstract】 The content of triterpenes extracted from sclerotia of Poria cocos was determined by colorimetry with vanillin-glacial acetic acid and perchloric acid,and the in vitro antibacterial activity of triterpenes was tested by plate growth rate method.The positive results of chemical reactions indicated that methanol extract of sclerotia of Poria cocos contains triterpenes and the content is 1.58%.Its lowest antibacterial concentrations are 0.47,0.47,0.95μg/ml against Escherichia coli,Staphylococcus aureusm,and Pseudomonas aeruginosa respectively.The results also showed that the inhibitory activities of this sclerotia extract against Escherichia coli and Staphylococcus aureusm are relatively stable and hardly affected by time.In conclusion,the triterpenes extracted from sclerotia of Poria cocos have good antimicrobial activity and could be further developed as drug of treating suppurative infection and natural cosmetic of dispelling blain.更多还原