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蛹虫草胞外锌多糖抗氧化能力的研究

Studies on the Antioxidant Capacity of Zinc Rich Exopolysaccharide of Cordyceps militaris

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【作者】 陈宏伟陈安徽邵颖樊美珍

【Author】 Chen Hongwei1,2,Chen Anhui2,Shao Ying1,2,Fan Meizhen11(Anhui Provincial Key Laboratory of Microbial Control,Anhui Agricultural University,Hefei 230036,China)2(Food and Biological Engineering School,Xuzhou Institute of Technology,Xuzhou 221008,China)

【机构】 安徽农业大学安徽省微生物防治重点实验室徐州工程学院食品(生物)工程学院

【摘要】 以蛹虫草为材料通过液体深层富锌培养获得胞外富锌多糖,测定了蛹虫草胞外富锌多糖的锌含量和抗氧化性能,即清除氧自由基、羟自由基和DPPH的能力。实验结果表明:蛹虫草富锌培养基内ZnSO4浓度在150μg/mL下,可以显著提高胞外锌多糖的有机锌含量(P<0.01),使有机锌含量达到0.87mg/g,比对照提高295%。有机锌对提高胞外锌多糖清除自由基的能力有显著的促进作用(P<0.01),清除氧自由基、羟自由基和DPPH的能力分别是对照的7.05%、23.5%和17.49%;胞外锌多糖对自由基的清除能力与多糖浓度呈明显的剂量-效应关系(P<0.01)。

【Abstract】 Zinc-rich exopolysaccharide was obtained by submerged fermentation of enriched zinc from Cordyceps militaris mycelium.The content of organic zinc and antioxidant capacity of zinc-rich exopolysaccharide was determined.The experiments showed that the organic zinc reaches 0.87 mg/g when the concentration of ZnSO4 in culture medium is 150 μg/mL(P<0.01),which was 295 % higher than those of the control.The organic zinc could greatly enhance the free radical scavenging capacities of zinc-rich exopolysaccharide(P<0.01).Its capacities of scavenging oxygen free radicals,hydroxyl radicals and DPPH were 7.05 %,23.5 % and 17.49 % higher than the control,respectively.The capacity to scavenge free radial displayed a dose-response relationship with the concentration of zinc-rich exopolysaccharide.

【基金】 江苏省“六大人才高峰”资助项目;江苏省“333工程”资助项目;江苏省高校科研计划指导项目(05KJD180203)
  • 【文献出处】 食品与发酵工业 ,Food and Fermentation Industries , 编辑部邮箱 ,2009年06期
  • 【分类号】R285
  • 【被引频次】37
  • 【下载频次】437
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