【摘要】 分别采用分光光度法、菌体量法以及孔扩散法研究了ε-聚赖氨酸对细菌和真菌的抑菌活性及ε-聚赖氨酸浓度、pH值和温度对其抑菌活性的影响。结果表明,ε-聚赖氨酸对金黄色葡萄球菌、大肠杆菌、黄曲霉、枯草芽孢杆菌、酿酒酵母、保加利亚乳杆菌等6种供试微生物均有一定的抑制作用,对大肠杆菌、金黄色葡萄球菌、保加利亚乳杆菌抑制效果较好,对黄曲霉较差;对各种菌的最小抑菌浓度(MIC)为大肠杆菌、金黄色葡萄球菌、保加利亚乳杆菌12.5 mg/L;枯草芽孢杆菌25 mg/L;酿酒酵母800 mg/L;黄曲霉1 600 mg/L。ε-聚赖氨酸的抑菌活性随其浓度的增加而增强,热稳定性非常好,能耐100℃的高温,在pH5～8抑菌活性最强,与甘氨酸、Nisin都有协同增效的作用。更多还原

【Abstract】 The anti-microbial activity of ε-Polylysine and its effect factors(including concentration,pH value,temperature) were studied by spectrophotometry,dry bacteria cell weight and aperture pervasion method.The results showed that ε-Polylysine had a inhibition function on Staphylococcus aureus,Escherichia coli,Aspergillus flavus,Bacillus subtilis,Saccharomyces cerevisiae and Lactobacillus bulgaricus,the antimicrobial action was especially notable to Escherichia coli,Staphylococcus aureus and Lactobacillus bulgaricus,and it was not very effective to Aspergillus flavus.The minimal inhibitory concentration(MIC) was 12.5mg/L for Escherichia coli,Staphylococcus aureus and Lactobacillus bulgaricus,25 mg/L for Bacillus subtilis,800mg/L for Saccharomyces cerevisiae,1 600mg/L for Aspergillus flavus.And it was proved that the stronger the concentration of ε-PL is,the stronger the anti-microbial activity is.It was also proved that ε-Polylysine has very good thermal stability,being able to endure as high as 100℃ temperature and it can be effective from pH5~8.Besides,the addition of glycine or Nisin can improve antimicrobial activity of ε-Polylysine.更多还原