【摘要】 目的探讨甲醛诱导DNA-蛋白质交联作用及其修复效应。方法采用培养V79细胞株作为实验材料,以不同浓度的液态甲醛(0、50、100、200、400、800、1 600μmol/L)对细胞染毒1 h后检测DPC率,并根据实验结果选取200μmol/L对培养细胞株进行修复实验(修复0、6、12、18、24 h)。采用KCl-SDS沉淀法检测DPC率。结果较高浓度的液态甲醛(≥200μmol/L)可以产生明显的DPC效应(P<0.05);由200μmol/L浓度的液态甲醛诱导V79细胞产生明显的DPC(P<0.05),经过修复可以恢复到空白对照水平(P>0.05)。结论较高浓度的甲醛可以明显诱导DPC形成,甲醛所致的DNA-蛋白质交联可以得到修复。更多还原

【Abstract】 Objective To explore the effect of formaldehyde(FA)on DNA-protein crosslink(DPC)and its repair.Methods V79 cell line from Chinese hamster was used in the study.The cells were cultured with different concentrations of FA solutions(0,50,100,200,400,800,1 600 μmol/L)for 1 h.DPC rate in V79 cell line was detected by KCl-SDS assay,the repair of DPC in 200 μmol/L FA solution was examined at 0,6,12,18 and 24 h respectively after exposure.Results The DPC in cells exposed to higher concentrations of FA solution(≥200 μmol/L)were significantly higher than those of control group(P<0.05),while the DPC induced by 200 μmol/L of FA solution could be repaired after 18 h and restored to control level after 24 h.Conclusions The results suggested that higher concentration of FA might induce DNA-protein crosslink,however this kind of DPC could be well repaired.更多还原