【摘要】 目的:采用大鼠腹腔注射脂多糖(LPS)复制大鼠急性肺损伤(ALI)模型,评价前列腺素E1脂微球制剂(凯时)对急性肺损伤大鼠的保护作用。方法:雄性健康Sprague Dawley大鼠60只随机分为3组,A组:生理盐水组;B组:LPS模型组;C组:凯时治疗组。动物以硫喷妥纳腹腔注射麻醉,于实验3h分别测量每只大鼠的肺系数,肺通透指数,肺湿重/肺干重,肺组织中髓过氧化物酶(MPO)含量,丙二荃(MDA)和过氧化物歧化酶(SOD)的含量,血清细胞因子TNF-a、IL-12、IL10的浓度,肺组织中NF-KB蛋白的表达,并进行统计学分析。结果:①B组与A组比较,肺系数,肺通透指数,肺湿重/肺干重显著增加(P<0.05);血浆SOD含量,血清细胞因子IL10显著减少(P<0.05);肺组织中髓过氧化物(MPO)活性和丙二荃(MDA)含量,血清细胞因子TNF-a,IL-12显著增加(P<0.05)。②C组与B组比较,肺系数,肺通透指数,肺湿重/肺干重显著减少(P<0.05);肺组织SOD含量,血清细胞因子IL10显著升高(P<0.05);肺组织中髓过氧化物(MPO)活性和丙二荃(MDA)含量,血清细胞因子TNF-a,IL-12显著减少(P<0.05)。③C组与B组比较,NF-kB P65的表达明显低于A组(P<0.05)。结论:前列腺素E1脂微球制剂通过抑制NF-kB的表达,减少中性粒细胞的渗出,对LPS诱导的急性肺损伤大鼠有明显保护作用。更多还原

【Abstract】 Objective: To investigate the protective effects of lipo-PGE1 on acute lung injury injury induced by lipopolysaccharied(LPS) in rats.Methods: In a All model by injected LPS through abdominal cavity,Sprague Dawley rats weighing 180g~220g were randomly devided into 3 groups with twenty each: group A(normal group,n=20),group B(LPS group,n=20),group C(LPS+lipo-PGE group).Lung index、Pulmonary microvasular permeability index,Wet/Dry weight,MPO,MDA,SOD,TNFA,IL12,IL10 were measured and statistically analyzed.Results: ① As compared with the normal group.Lung index,Pulmonary microvasular permeability index,Wet/Dry weight increased significantly(P<0.05);SOD,IL10 level decreased singnificantly(P<0.05);MPO,MDA,SOD,TNFA,IL12 increased singnificantly(P<0.05)in the acute lung injury group.② Lung index,Pulmonary microvasular permeability index,Wet/Dry weight were significantly lower(P<0.05);SOD,IL10 level were significantly higher(P<0.05);MPO,MDA,SOD,TNFA,IL12 were markedly lower(P<0.05)in lipo-PGE1 group than those in LPS-inducde group.③ A significant decrease was in the phosphorylation of Ser536 on NF-kB observed in the lipo-PGE1 group,compared with the acute lung injury grou(P<0.05).Conclusion: lipo-PGE1 could inhibit expression of NF-kB and reduce exudation of PMNs,and had protective effects on acute lung injury induced by LPS in rats.更多还原